Affinity Chromatography. New Approaches for the Preparation of Spacer Containing Derivatives and for Specific Isolation of Peptides

  • Meir Wilchek
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)


Affinity chromatography is a method for purification of biologically active compounds by virtue of biological recognition between certain ligands and proteins (1,2,3). Figure 1 shows a general scheme for purification of compounds by affinity chromatography. Purification is achieved by chromatography of a mixture containing the protein to be purified on a column of an insoluble matrix to which a specific ligand has been covalently bound. Proteins and other molecules not exhibiting appreciable affinity for the ligand will pass unretarded through the column, whereas those which “recognize” the ligand will be retarded. Elution of the bound protein, is readily achieved by changing the medium to conditions unfavourable for combination.


Affinity Chromatography Cyanogen Bromide FEBS Letter Label Peptide Dansyl Chloride 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Meir Wilchek
    • 1
  1. 1.Department of BiophysicsThe Weizmann Institute of ScienceRehovotIsrael

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