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Affinity Chromatography. New Approaches for the Preparation of Spacer Containing Derivatives and for Specific Isolation of Peptides

  • Meir Wilchek
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)

Abstract

Affinity chromatography is a method for purification of biologically active compounds by virtue of biological recognition between certain ligands and proteins (1,2,3). Figure 1 shows a general scheme for purification of compounds by affinity chromatography. Purification is achieved by chromatography of a mixture containing the protein to be purified on a column of an insoluble matrix to which a specific ligand has been covalently bound. Proteins and other molecules not exhibiting appreciable affinity for the ligand will pass unretarded through the column, whereas those which “recognize” the ligand will be retarded. Elution of the bound protein, is readily achieved by changing the medium to conditions unfavourable for combination.

Keywords

Affinity Chromatography Cyanogen Bromide FEBS Letter Label Peptide Dansyl Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    CUATRECASAS, P., WILCHEK, M. & ANFINSEN, C.B., Proc. Nat. Acad. Sci.U.S.A. 61:636 (1968).CrossRefGoogle Scholar
  2. 2.
    WILCHEK, M. Biochem.J. 127; 7p (1972).Google Scholar
  3. 3.
    WILCHEK, M. & GIVOL, D. “Peptides 1971”, North-Holland Publishing Co., 203 (1973).Google Scholar
  4. 4.
    CUATRECASAS, P. & WILCHEK, M. Biochem. Biophys. Res. Commun. 33: 225 (1968).CrossRefGoogle Scholar
  5. 5.
    CUATRECASAS, P. J. Biol. Chem. 245:3059 (1970).Google Scholar
  6. 6.
    WILCHEK, M., & ROTMAN, M. Israel J. Chem. 8:172p (1970).Google Scholar
  7. 7.
    WALSH, D.A., PERKINS, J.P. & KREBS, E.G. J.Biol.Chem. 243:3763 (1968).Google Scholar
  8. 8.
    CORBIN, J.D., REIMANN, E.M., WALSH, D.A. & KREBS, E.G. J. Biol. Chem. 245:4849 (1970).Google Scholar
  9. 9.
    WILCHEK, M., SALOMON, Y., LOWE, M. & SELINGER, Z. Biochem.Biophys. Res. Commun. 45:1177 (1971).CrossRefGoogle Scholar
  10. 10.
    TESSER, G.I., FISCH, H.U. & SCHWYZER, R. FEBS Letters 23:56 (1972).CrossRefGoogle Scholar
  11. 11.
    SVENSSON, B. FEBS Letters 29:167 (1973).CrossRefGoogle Scholar
  12. 12.
    AXEN, R. & ERNBACK, S. Eur. J. Biochem. 18:351 (1971).CrossRefGoogle Scholar
  13. 13.
    WILCHEK, M. FEBS Letters 33:70 (1973).CrossRefGoogle Scholar
  14. 14.
    WILCHEK, M. Proceedings of the Ninth International Congress of Biochemistry. Stockholm, p.9 (1973).Google Scholar
  15. 15.
    ER-EL, Z., ZAIDENZAIG, Y. & SHALTIEL, S. Biochem. Biophys. Res. Commun. 49:383 (1972).CrossRefGoogle Scholar
  16. 16.
    SHALTIEL, S. & ER-EL, Z. Proc. Nat. Acad. Sci. U.S.A. 70:778 (1973).CrossRefGoogle Scholar
  17. 17.
    HOFSTEE, B.H.J. Biochem. Biophys. Res. Commun. 50:751 (1973).CrossRefGoogle Scholar
  18. 18.
    LAMED, R., LEVIN, Y. & WILCHEK, M. Biochim. Biophys. Acta 304: 231 (1973).CrossRefGoogle Scholar
  19. 19.
    WILCHEK, M. & LAMED, R. Methods in Enzymology, 1974. Volume edited by W.Jakoby and M. Wilchek (in press).Google Scholar
  20. 20.
    LAMED, R., LEVIN, Y. & OPLATKA, A. Biochim. Biophys. Acta 303: 163 (1973).Google Scholar
  21. 21.
    SINGER, S.J. Adv. Prot. Chem. 22:1 (1967).CrossRefGoogle Scholar
  22. 22.
    VALLEE, B.L. & RIORDAN, J.F. Ann. Rev. Biochem. 38:733 (1969).CrossRefGoogle Scholar
  23. 23.
    SHAW, E. Physiol. Rev. 50:244 (1970).Google Scholar
  24. 24.
    COHEN, L. Ann. Rev. Biochem. 37 (1968).Google Scholar
  25. 25.
    GIVOL, D., WEINSTEIN, Y., GORECKI, M. & WILCHEK, M. Biochem. Biophys. Res. Commun. 38:827 (1970).CrossRefGoogle Scholar
  26. 26.
    WILCHEK, M. FEBS Letters, 7:161 (1970).CrossRefGoogle Scholar
  27. 27.
    GIVOL, D., STRAUSBAUCH, P.H., HURWITZ, E., WILCHEK, M., HAIMOVICH, J. & EISEN, M.N. Biochemistry 10:3461 (1971).CrossRefGoogle Scholar
  28. 28.
    GORECKI, M., WILCHEK, M. & PATCHORNIK, A. Israel J.Chem. 8: 173p (1970).Google Scholar
  29. 29.
    WILCHEK, M., BOCCHINI, V., BECKER, M. & GIVOL, D. Biochemistry 10:2828 (1971).CrossRefGoogle Scholar
  30. 30.
    MURDOCK, A.L., GRIST, K.L. & HIRS, C.H.W. Arch.Biochem. Biophys. 114:375 (1966).CrossRefGoogle Scholar
  31. 31.
    SCOFFONE, E., FONTANA, A. & ROCCHI, R. Biochemistry 7:971 (1968).CrossRefGoogle Scholar
  32. 32.
    WILCHEK, M. & MIRON, T. Biochim.Biophys. Acta 278:1 (1972).Google Scholar
  33. 33.
    WILCHEK, M. & MIRON, T. Biochem. Biophys. Res. Commun. 47:1015 (1972).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Meir Wilchek
    • 1
  1. 1.Department of BiophysicsThe Weizmann Institute of ScienceRehovotIsrael

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