Lactase Immobilized on Stainless Steel and Other Dense Metal and Metal Oxide Supports

Abstract

The past decade has seen the development of a large number of techniques for immobilizing enzymes which have been reviewed and discussed at length elsewhere(1,2,3). For the present purposes we consider those techniques involving the attachment of enzymes to particulate supports by means other than physical entrapment. While a number of methods have been described, (1,2,3), covalent binding seems to have gained the most favor. Catalysts prepared by such techniques have typically been used in packed bed reactors to process what might be referred to as “reagent grade” substrate streams.