Advertisement

Immobilized Cofactors and Multi-Step Enzyme-Systems

  • Klaus Mosbach
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)

Abstract

Immobilized cofactors or coenzymes are of interest for two major reasons. They show potential as active immobilized coenzymes when used in enzymic processes such as steroid transformation (1); they find use as ligands in biospecific affinity chromatography. The various cofactor analogues synthesized in our laboratory and immobilized to Sepharose include: ε-aminohexanoyl-NAD+ (2), NAD+- N6[N-(6-aminohexyl)-acetamide], and N6-carboxy-methyl-NAD+ (3), N6-(6-aminohexyl)-AMP (4–6) and 8-(6-aminohexyl)-aminocyclic AMP (7).

Keywords

Affinity Chromatography Immobilize Enzyme Enzymic Process Polymer Bead FEBS Letter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Mosbach, K. and Larsson, P. O. (1970) Biotechn. & Bioeng. Vol. XII, 1927.Google Scholar
  2. 2.
    Larsson, P. O. and Mosbach, K. (1971) Biotechn. & Bioeng. Vol. XIII, No. 3, 393–398.Google Scholar
  3. 3.
    Lindberg, M., Larsson, P. O. and Mosbach, K., Eur. J. Biochem. in press.Google Scholar
  4. 4.
    Mosbach, K., Guilford, H., Larsson, P. O., Ohlsson R. and Scott, M. (1972) Biochem. J. 125, No. 2, 20–21.Google Scholar
  5. 5.
    Guilford, H., Larsson, P. O. and Mosbach, K. (1972) Chemica Scripta, 2, 165–170.Google Scholar
  6. 6.
    Mosbach, K., Guilford, H., Ohlsson, R. and Scott, M. (1972) Biochem. J. 127, 625–631.Google Scholar
  7. 7.
    Jergil, B., Guilford, H. and Mosbach, K., Biochem. J. in press.Google Scholar
  8. 8.
    Ohlsson, R., Brodelius, P. and Mosbach, K. (1972) FEBS Letters, 25, No. 2, 234–238Google Scholar
  9. 9.
    Dixon, J. E., Everse, J., Lee, C. Y., Taylor, S. S., Kaplan, N. O. and Mosbach, K., to be published.Google Scholar
  10. 10.
    Lowe, C. R., Mosbach, K. and Dean, P. (1972), Biochem. & Biophys. Res. Comm., 48, No. 4, 1004–1010.Google Scholar
  11. 11.
    Brodelius, P. and Mosbach, K. (1973) FEBS Letters, 35, No. 2, 223–226.Google Scholar
  12. 12.
    Mosbach, K., and Mattiasson, B., (1970) Acta Chem. Scand. 24, 2093–2100.CrossRefGoogle Scholar
  13. 13.
    Mattiasson, B., and Mosbach, K., (1971) Biochim. et Biophys. Acta, 235, 253–257.Google Scholar
  14. 14.
    Srere, P. A., Mattiasson, B. and Mosbach, K., (1973) Proc. Nat. Acad. Sci., 70, No. 9 2534–2538.CrossRefGoogle Scholar
  15. 15.
    Gestrelius, S., Mattiasson, B. and Mosbach, K. (1972) Biochim. et Biophys. Acta, 276, 339–343.Google Scholar
  16. 16.
    Gestrelius, S., Mattiasson and Mosbach, K. (1973) Eur. J. Biochem. 36, 89–96.CrossRefGoogle Scholar
  17. 17.
    Nilsson, H., Akerlund, A. C. and Mosbach, K. (1973) Biochim. et Biophys. Acta, 320, 529–534.Google Scholar
  18. 18.
    Nilsson, H., Mosbach, R. and Mosbach, K. (1972) Biochim. et Biophys. Acta, 268, 253–256.Google Scholar
  19. 19.
    Johansson, A., Lundberg, J., Mattiasson, B. and Mosbach, K. (1973) Biochim. et Biophys. Acta 304, 217–221.Google Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Klaus Mosbach
    • 1
  1. 1.Biochemical DivisionChemical Center University of LundLund, 7Sweden

Personalised recommendations