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Immobilized Polynucleotides and Nucleic Acids

  • P. T. Gilham
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)

Abstract

The use of immobilized nucleic acids and polynucleotides in the study of nucleic acids and their associated enzymes has become widespread in recent years. There are two main areas of application: the fractionation of nucleic acids and polynucleotides through base-paired complex formation and the isolation and purification of nucleic acid-associated enzymes by affinity chromatography. There are now a number of methods for the preparation of immobilized polynucleotides and the potential uses of these materials depend to some extent on the means by which the polymers are attached to the insoluble supports.

Keywords

Covalent Linkage Cyanogen Bromide Support Matrix Physical Entrapment tRNA Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • P. T. Gilham
    • 1
  1. 1.Department of Biological SciencesPurdue UniversityWest LafayetteUSA

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