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Affinity Chromatography — Old Problems and New Approaches

  • Steven C. March
  • Indu Parikh
  • Pedro Cuatrecasas
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)

Abstract

The purification of macromolecules by biospecific adsorption-affinity chromatography (1) is a technique with a long history although its application to a wide variety of problems is comparatively recent. As long ago as 1910 there were reports of the purification of amylase by adsorption to insoluble starch (2). Tyrosinase was purified in 1953 using a cellulose matrix bearing the inhibitor, diazodizine (3). In both of these examples the power of purification lies in the highly selective “insolubilization” of the macromolecule from solution. This reversible phase separation often allows a high degree of concentration of the particular molecular species of interest from dilute solution and the removal, by washing and elution processes, of other molecular species that are cosolutes.

Keywords

Affinity Chromatography Cyanogen Bromide Reversible Phase Separation Aleutian Mink Disease Virus Affinity Chroma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Steven C. March
    • 1
  • Indu Parikh
    • 1
  • Pedro Cuatrecasas
    • 1
  1. 1.Johns Hopkins University School of MedicineBaltimoreUSA

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