Mutation Causing Temperature-Sensitive Expression of Cell Transformation by a Tumor Virus

  • Hartmut C. Renger
  • Claudio Basilico
Part of the Milestones in Current Research book series (MCR)


A procedure has been devised to isolate 3T3 mouse fibroblasts transformed by simian virus 40 (SV40) that express their transformed phenotype at low (32°C) but not at high (39°C) temperature. Three parameters typical of malignant growth in vitro: (a) high saturation density in culture, (b) ability to form colonies on monolayers of normal 3T3 cells, and (c) lack of contact inhibition of DNA synthesis, are temperature sensitive. These phenotypic changes are fully reversible. The serum requirement for growth appears to be largely unchanged by temperature. These cells seem to owe their behavior to a cellular, rather than to a viral, alteration since after fusion of the temperature-sensitive transformed cells with permissive monkey cells, a procedure that leads to rescue (i.e., multiplication of the virus), wild-type SV40 virus is produced.


Contact Inhibition Saturation Density Polyoma Virus SV3T3 Cell High Saturation Density 
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SV40-transformed 3T3 cells


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© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Hartmut C. Renger
  • Claudio Basilico

There are no affiliations available

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