Abortive Transformation by the Tsa Mutant of Polyoma Virus

  • Renato Dulbecco
  • Michael Stoker
Part of the Milestones in Current Research book series (MCR)


The Tsa mutant of polyoma virus (PyTsa) is a conditional lethal mutant isolated by Fried1. PyTsa grows normally at the permissive temperature (31.5° C), but at the nonpermissive temperature (38.5° C) there is a failure of normal viral DNA synthesis, and of vegetative growth in mouse cells. Transformation of hamster cells by PyTsa is also inhibited at 38.5° C; this raises the question, Is the mutant gene required simply for the viral induced change in the cell physiology or is it concerned with the stable propagation of the transformed state? These alternatives can be distinguished by the phenomenon of abortive transformation2.


Vegetative Growth BHK21 Cell Wild Type Virus Permissive Temperature Polyoma Virus 
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  1. 1.
    Fried, M., Virology, 25, 669 (1965).PubMedCrossRefGoogle Scholar
  2. 2.
    Stoker, M., Nature, 218, 234 (1968).PubMedCrossRefGoogle Scholar
  3. 3.
    Stoker, M., O’Neill, C., Berryman, S., and Waxman, V., Int. J. Cancer, 3, 688 (1968).CrossRefGoogle Scholar
  4. 4.
    Crawford, L. V., Virology, 18, 177 (1962).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Renato Dulbecco
  • Michael Stoker

There are no affiliations available

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