A Map of Simian Virus 40 Transcription Sites Expressed in Productively Infected Cells
The topographical location of “early” and “late” sequences on the physical map of the simian virus 40 genome was determined by reacting unlabeled RNA from monkey cells productively infected with simian virus 40 with the separated strands of each of the 11 DNA fragments formed by digesting supercoiled virus DNA with the restriction endonuclease of Hemophilus influenzae. Stable species of early RNA are complementary to the minus strands of the contiguous fragments A, H, I and B, while the late RNA is transcribed predominantly from the plus strands of fragments A, C, D, E, K, F, J, G and B, which also form a continuous set on the physical map. This result is in agreement with previous findings, which indicated that transcription before DNA synthesis is confined to the minus DNA strand, while late transcription includes sequences located predominantly on the plus strand.
KeywordsSimian Virus Minus Strand Late Transcription Separate Strand Half Molecule
simian virus 40
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