Strand Orientation of Simian Virus 40 Transcription in Productively Infected Cells

  • Dona M. Lindstrom
  • Renato Dulbecco
Part of the Milestones in Current Research book series (MCR)


Strand orientation of transcription in BSC-1 cells infected by simian virus 40 (SV40) was investigated by annealing RNAs extracted from infected cells with asymmetric complementary RNA from SV40 DNA synthesized in vitro by RNA polymerase from Escherichia coli. The results suggest that the early viral RNA sequences (those made before replication of viral DNA) and the late RNA sequences (those synthesized concurrently with replication of viral DNA) are transcribed from opposite strands of the SV40 DNA. The RNA synthesized in vitro is of the same polarity as the early in vivo RNA and is complementary to the late in vivo RNA. Knowledge of strand selection in lytic infection is a step towards understanding the regulation of transcription of this viral DNA.


Simian Virus Lytic Infection Strand Orientation RNase Digestion Glass Fiber Disc 
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complementary RNA


standard saline citrate, 0.15 M sodium chloride-0.015 M sodium citrate


simian virus 40


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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Dona M. Lindstrom
  • Renato Dulbecco

There are no affiliations available

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