Advertisement

Regulation of Transcription of the SV40 DNA in Productively Infected and in Transformed Cells

  • Kinichiro Oda
  • Renato Dulbecco
Part of the Milestones in Current Research book series (MCR)

Abstract

The expression of genes of the oncogenic virus SV40 is regulated. In productive infection, some genes are expressed before viral DNA replication begins (“early” genes); others are expressed after it has begun (“late” genes).1 In transformed cells, which contain the complete viral genome,2 some genes are expressed; others are not. Whether either type of regulation is at the level of transcription of viral genes is the question raised in the work to be reported here. The basic technique was hybridization of RNA synthesized in infected or transformed cells to viral DNA, and hybridization competition experiments. Results partly similar to those reported here were recently published by Aloni et al.3

Keywords

Sodium Dodecyl Sulfate Cytoplasmic Fraction Standard Saline Citrate African Green Monkey Kidney Cell Polysome Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

  1. 1.
    Rapp, F., J. L. Melnick, and T. Kitahara, Science, 147, 625 (1965).PubMedCrossRefGoogle Scholar
  2. 2.
    Koprowski, H., F. C. Jensen, and Z. Steplewski, these Proceedings, 58, 127 (1967)Google Scholar
  3. Watkins, J. F., and R. Dulbecco, these Proceedings, 58, 1396 (1967).Google Scholar
  4. 3.
    Aloni, Y., E. Winocour, and L. Sachs, J. Mol. Biol., 31, 415 (1968).PubMedCrossRefGoogle Scholar
  5. 4.
    Attardi, B., and G. Attardi, these Proceedings, 58, 1051 (1967).Google Scholar
  6. 6.
    Marmur, J., J. Mol. Biol., 3, 208 (1961).CrossRefGoogle Scholar
  7. 6.
    Oda, K., and W. K. Joklik, J. Mol. Biol., 27, 395 (1967).PubMedCrossRefGoogle Scholar
  8. 7.
    Gillespie, D., and S. Spiegelman, J. Mol. Biol., 12, 829 (1965).PubMedCrossRefGoogle Scholar
  9. 8.
    Gershon, D., L. Sachs, and E. Winocour, these Proceedings, 56, 918 (1966).Google Scholar
  10. 9.
    Blobel, G., and V. R. Potter, J. Mol. Biol., 26, 293 (1967).PubMedCrossRefGoogle Scholar
  11. 10.
    Baum, S. G., P. R. Reich, C. J. Hybner, W. P. Rowe, and S. M. Weissman, these Proceedings, 56, 1509 (1966).Google Scholar
  12. 12.
    Rowe, W. P., and S. G. Baum, these Proceedings, 52, 1340 (1964)Google Scholar
  13. Rapp, F., J. L. Melnick, J. S. Butel, and T. Kitahara, these Proceedings, 52, 1348 (1964)Google Scholar
  14. Black, P. H., and G. Todaro, these Proceedings, 54, 374 (1965).Google Scholar
  15. 13.
    Hatanaka, M., and R. Dulbecco, these Proceedings, 58, 1888 (1967).Google Scholar

Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Kinichiro Oda
  • Renato Dulbecco

There are no affiliations available

Personalised recommendations