Measuring Sedimentation, Diffusion, and Molecular Weights of Small Molecules by Direct Fitting of Sedimentation Velocity Concentration Profiles
Sedimentation velocity experiments have traditionally been used for samples with relatively high sedimentation coefficients and low diffusion. Such samples give sharp boundaries from which it is relatively easy to extract the sedimentation coefficient, and which permit the separation of multicomponent samples into distinct boundaries. However, many proteins of interest for therapeutic purposes, such as cytokines and growth factors, have molecular masses of only 10–40 kDa. Even at 60000 rpm, such small molecules give very broad boundaries which are difficult to analyze by existing techniques.
KeywordsSedimentation Velocity Sedimentation Coefficient Cell Bottom Partial Specific Volume Derivative Data
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