Analysis of Protein-Nucleic Acid and Protein-Protein Interactions Using Multi-Wavelength Scans from the XL-A Analytical Ultracentrifuge
Protein-nucleic acid and protein-protein interactions have been studied by a variety of biochemical and physicochemical techniques, each of which has distinctive advantages and disadvantages. In particular, analytical ultracentrifugation has the advantages that it is rigorously based upon reversible thermodynamics, and the reactants and product or products of an interaction each have uniquely defined concentration gradients that can be resolved by appropriate mathematical analysis to give the desired values of the natural logarithms of the equilibrium constants with a minimum of assumptions.
KeywordsExtinction Coefficient Molar Extinction Coefficient Radial Position Heat Shock Factor Absorbance Unit
Unable to display preview. Download preview PDF.
- Durchschlag H (1986): Specific volumes of biological macromolecules and some other molecules of biological interest. In: Thermodynamic Data for Biochemistry and Biotechnology., Hinz, H. J, ed. New York: Springer—Verlag.Google Scholar
- Jansen DE, Kelly RC and von Hippel PH (1976): DNA “Melting” Proteins. II Effects on bacteriophage T4 gene 32-protein on the conformation and stability of nucleic acid structures. J Biol Chem 251: 7215–7228Google Scholar
- Lewis, MS (1991): Ultracentrifugal analysis of a mixed association. Biochemistry 30: 11716–11719Google Scholar
- Lewis MS, Shrager RI and Kim S-J (1994): Ultracentrifugal analysis of protein—nucleic acid interactions using multi—wavelength scans. Colloid and Polymer Sci: in press.Google Scholar
- Ross JBA, Senear DF, Waxman E, Kombo BB, Rusinova E, Huang YT, Laws WR and Hasselbacher CA (1992): Spectral enhancement of proteins: Biological incorporation and fluorescence characterization of 5-hydroxy tryptophan in bacteriophage cl repressor. Proc Nat Acad Sci USA 89: 12023–12027PubMedCrossRefGoogle Scholar
- Strang G (1986) Introduction to Applied Mathematics. Wellesley, MA: Wellesley—Cambridge Press. pp 138–139Google Scholar