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Analysis of Protein-Nucleic Acid and Protein-Protein Interactions Using Multi-Wavelength Scans from the XL-A Analytical Ultracentrifuge

  • Marc S. Lewis
  • Richard I. Shrager
  • Soon-Jong Kim
Part of the Emerging Biochemical and Biophysical Techniques book series (EBBT)

Abstract

Protein-nucleic acid and protein-protein interactions have been studied by a variety of biochemical and physicochemical techniques, each of which has distinctive advantages and disadvantages. In particular, analytical ultracentrifugation has the advantages that it is rigorously based upon reversible thermodynamics, and the reactants and product or products of an interaction each have uniquely defined concentration gradients that can be resolved by appropriate mathematical analysis to give the desired values of the natural logarithms of the equilibrium constants with a minimum of assumptions.

Keywords

Extinction Coefficient Molar Extinction Coefficient Radial Position Heat Shock Factor Absorbance Unit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Boston 1994

Authors and Affiliations

  • Marc S. Lewis
  • Richard I. Shrager
  • Soon-Jong Kim

There are no affiliations available

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