The Sedimentation Equilibrium Analysis of Polysaccharides and Mucins: A Guided Tour of Problem Solving for Difficult Heterogeneous Systems

  • Stephen E. Harding
Part of the Emerging Biochemical and Biophysical Techniques book series (EBBT)


For the last 12 years we have been applying hydrodynamic methods to study a particularly difficult heterogeneous class of biological macromolecule - the polysaccharides - which possibly present the ultracentrifuge with one of its biggest challenges (see, e.g. Harding, 1992). In this paper I am going to describe some of the many problems we’ve encountered in trying to use sedimentation equilibrium to study the size, size distribution and interactions of these molecules and, in particular, a particular class of polysaccharide-protein conjugate known as the “mucopolysaccharides” or “mucins” (Harding, 1989).


Molecular Weight Distribution Virial Coefficient Sedimentation Equilibrium Molecular Weight Analysis Fringe Number 
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© Birkhäuser Boston 1994

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  • Stephen E. Harding

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