Calcium Phosphate-Mediated DNA Transfection

  • Patricia L. Chang


The ability to express foreign genes introduced into cultured cells is a powerful tool for studying gene expression. It permits monitoring not only the transcriptional and translational activities of exogenous DNA but also the regulation of gene expression by other genetic elements. Furthermore, the approach to somatic gene therapy is entirely based on introducing genes either to correct a prior genetic defect or to augment the genetic repertoire of the recipient cell. The technology of gene transfer is truly one of the cornerstones in molecular genetics.


Transient Expression Thymidine Kinase Endocytic Pathway Stable Integration Human Primary Fibroblast 
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© Birkhäuser Boston 1994

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  • Patricia L. Chang

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