Detection of Specific mRNAs by In Situ Hybridization
All glassware should be baked at ⊖ 200°C overnight All solutions, except those containing Tris, should be treated with diethylpyrocarbonate (Sigma, D-5758) for at least 2 hr and then autoclaved. Gloves should be worn throughout.
KeywordsDextran Sulfate Specific mRNAs Saline Sodium Citrate Enzyme Buffer Autoradiography Emulsion
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