Advertisement

Commercial Liposomes and Electroporation Can Deliver Soluble Antigen for Class I Presentation in CTL Generation

  • Weisan Chen
  • James McCluskey

Abstract

Major histocompatibility complex (MHC) class I antigens present processed antigens derived almost exclusively from the cellular cytoplasm (see Chapter 5, this volume). Accordingly, extracellular soluble antigens are unusual targets for class I-restricted cytotoxic T lymphocytes (CTL) and little is known regarding mechanisms of self-tolerance to soluble antigens in the CD8+ T-cell compartment. In addition, vaccines designed to elicit CD8+ class I-restricted CTL usually require immunization with live attenuated virus, rather than with purified antigens. Such vaccines are contraindicated in patients who are immune-suppressed or who have a history of life-threatening hypersensitivity. Moreover, vaccination with attenuated viruses can cause postvaccine illness, which is undesirable in large vaccination programs. Therefore, strategies which might generate class I-restricted CTL by immunization with soluble (recombinant) protein would be very valuable in relation to practical vaccine development and in probing the scope of self-tolerance by CD8+ T lymphocytes.

Keywords

Spleen Cell Soluble Antigen Antigen Loading Hypotonic Challenge Dioleoyl Phosphatidylethanolamine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Harding CV, Collins DS, Kanagawa O, Unanue ER (1991): Liposome-encapsulated antigens engender lysosomal processing for class II presentation and cytosolic processing for class I presentation. J Immunol 147: 2860–2863Google Scholar
  2. Lambert H, Pankov R, Gauthier J, Hancock R (1990): Electroporation-mediated uptake of proteins into mammalian cells. Biochem Cell Biol 68: 729–734CrossRefGoogle Scholar
  3. Mir LM, Banoun H, Paoletti C (1988): Introduction of definite amounts of nonpermeant molecules into living cells after electropermeabilization: Direct access to the cytosol. Exp Cell Res 175: 15–25CrossRefGoogle Scholar
  4. Monte PD, Szoka JR (1989): Effect of liposome encapsulation on antigen presentation in vitro. J Immunol 142: 1437–1443Google Scholar
  5. Moore MW, Carbone FR, Bevan MJ (1988): Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell 54: 777–785CrossRefGoogle Scholar
  6. Nair S, Zhou F, Huang L, Reddy R, Huang L, Rouse BT (1992): Soluble proteins delivered to dendritic cells via pH-sensitive liposomes induce primary cytotoxic T lymphocyte responses in vitro. J Exp Med 175: 609–612CrossRefGoogle Scholar
  7. Reddy R, Zhou F, Huang L, Carbone F, Bevan M, Rouse BT (1991): pH sensitive liposomes provide an efficient means of sensitizing target cells to class I restricted CTL recognition of a soluble protein. J Immunol Methods 141: 157–163Google Scholar
  8. Zimmerman U (1982): Electric field mediated fusion and related phenomena. Biochim Biophys Acta 694: 227–277Google Scholar

Copyright information

© Birkhäuser Boston 1993

Authors and Affiliations

  • Weisan Chen
  • James McCluskey

There are no affiliations available

Personalised recommendations