Commercial Liposomes and Electroporation Can Deliver Soluble Antigen for Class I Presentation in CTL Generation
Major histocompatibility complex (MHC) class I antigens present processed antigens derived almost exclusively from the cellular cytoplasm (see Chapter 5, this volume). Accordingly, extracellular soluble antigens are unusual targets for class I-restricted cytotoxic T lymphocytes (CTL) and little is known regarding mechanisms of self-tolerance to soluble antigens in the CD8+ T-cell compartment. In addition, vaccines designed to elicit CD8+ class I-restricted CTL usually require immunization with live attenuated virus, rather than with purified antigens. Such vaccines are contraindicated in patients who are immune-suppressed or who have a history of life-threatening hypersensitivity. Moreover, vaccination with attenuated viruses can cause postvaccine illness, which is undesirable in large vaccination programs. Therefore, strategies which might generate class I-restricted CTL by immunization with soluble (recombinant) protein would be very valuable in relation to practical vaccine development and in probing the scope of self-tolerance by CD8+ T lymphocytes.
KeywordsSpleen Cell Soluble Antigen Antigen Loading Hypotonic Challenge Dioleoyl Phosphatidylethanolamine
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