Generation of Antigen-Specific Murine CTL Under Weakly Immunogenic Conditions

  • Sergey G. Apasov


The two-step generation of allospecific cytotoxic T cell lymphocytes (CTL) is an effective method to obtain highly active antigen-specific CTL. The first step involves the in vivo induction of CTL precursors (pCTL) followed by in vitro incubation of these precursors as the second step for the development of pCTL into CTL. The method was introduced (Wagner et al., 1976; Starzinski-Powitz et al., 1976) to produce CTL recognizing the hapten-conjugated syngeneic structures of the major histocompatibility complex (MHC). Later this approach was successfully used to develop CTL recognizing minor alloantigens (Czitrom and Gascoigne, 1983) or to generate CTL in the system where the donor and recipient differ by point mutations in MHC class I molecules (Brondz et al., 1987).


Major Histocompatibility Complex Mixed Lymphocyte Culture Pool Lymph Node Full Growth Medium Cell Separation Techni 
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  1. Brondz BD, Khan ES, Chervonsky AV, Isacova VR, Apasov SG, Blandova ZK (1987): Differential genetic requirement for in vivo and in vitro induction of T-killer and T-suppressor cells in the mutant H-2K6 system and the cross-reactivity of the T-killer clones. Exp Clin Immunogenet 4: 211–221Google Scholar
  2. Czitrom AA, Gascoigne NRJ (1983): Primary T-cell responses to minor alloantigens I. Characterization of cytotoxic effector cells generated from regional lymph nodes after immunization in the footpad. Immunology 50: 121–129Google Scholar
  3. Gascoigne NRJ, Crispe IN (1984): Differential expression of the repertoire in in vitro and in vivo responses to minor alloantigens. Immunogenetics 19: 511–517CrossRefGoogle Scholar
  4. Starzinski-Powitz A, Pfizenmaier K, Rollingoff M, Wagner H (1976): In vivo sensitization of T cells to hapten-conjugated syngeneic structures of major histocompatibility complex. I. Effect of in vitro culture upon generation of cytotoxic T lymphocytes. Eur J Immunol 6: 799–805CrossRefGoogle Scholar
  5. Wagner H, Starzinski-Powitz A, Pfizenmaier K, Rollingoff M (1976): Regulation of T cell-mediated cytotoxic allograft responses. II. Evidence for antigen-specific suppressor T cells. Eur J Immunol 6: 873–878CrossRefGoogle Scholar

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© Birkhäuser Boston 1993

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  • Sergey G. Apasov

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