Generation of CD4+ and CD8+ Antiinfluenza CTL and Assay of In Vitro Cytotoxicity

  • Vivian Lam Braciale


Mice that are primed in vivo at 6–12 weeks of age by immunizing intravenously with 100–300 hemagglutinating units (HAU) of virus in allantoic fluid are used as spleen donors three or more weeks afterwards. Immune spleen cells (100 × 106) are stimulated in vitro by co-culture with 20 × 106 virusinfected, irradiated (2000 rad) naive splenocytes in 75-cm2 flasks containing 40 ml of minimal essential medium (MEM) (GIBCO) plus 10% fetal bovine serum (FBS) and 5 × 10−5 m 2-mercaptoethanol (2-ME) (Braciale et al., 1982).


Major Histocompatibility Complex Allantoic Fluid Major Histocompatibility Complex Binding Immune Spleen Cell Peptide Major Histocompatibility Complex 
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Copyright information

© Birkhäuser Boston 1993

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  • Vivian Lam Braciale

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