SPDP Crosslinking of Antibodies to Form Heteroconjugates Mediating Redirected Cytotoxicity
Succinimidyl-3-(2-pyridyldithiol)-propionate (SPDP) is used to heterocrosslink two different antibodies or their Fab fragments. Crosslinking is through a disulfide bond, and is therefore reducible. Variations on this procedure crosslink antibodies via nonreducible thioether linkages.
KeywordsDisulfide Borate Dithiothreitol Iodoacetamide Maleimide
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