SPDP Crosslinking of Antibodies to Form Heteroconjugates Mediating Redirected Cytotoxicity

  • David M. Segal

Abstract

Succinimidyl-3-(2-pyridyldithiol)-propionate (SPDP) is used to heterocrosslink two different antibodies or their Fab fragments. Crosslinking is through a disulfide bond, and is therefore reducible. Variations on this procedure crosslink antibodies via nonreducible thioether linkages.

Keywords

Disulfide Borate Dithiothreitol Iodoacetamide Maleimide 

Reference

  1. Titus JA, Garrido MA, Hecht TT, Winkler DF, Wunderlich JR, Segal DM (1987): Human T cells targeted with anit-T3 crosslinked to anti-tumor antibody prevent tumor growth in nude mice. J Immunol 138: 4018–4022Google Scholar

Copyright information

© Birkhäuser Boston 1993

Authors and Affiliations

  • David M. Segal

There are no affiliations available

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