SPDP Crosslinking of Antibodies to Form Heteroconjugates Mediating Redirected Cytotoxicity

  • David M. Segal


Succinimidyl-3-(2-pyridyldithiol)-propionate (SPDP) is used to heterocrosslink two different antibodies or their Fab fragments. Crosslinking is through a disulfide bond, and is therefore reducible. Variations on this procedure crosslink antibodies via nonreducible thioether linkages.


Sodium Borate Prevent Tumor Growth Intact Antibody Thioether Bond Disulfide Exchange 
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  1. Titus JA, Garrido MA, Hecht TT, Winkler DF, Wunderlich JR, Segal DM (1987): Human T cells targeted with anit-T3 crosslinked to anti-tumor antibody prevent tumor growth in nude mice. J Immunol 138: 4018–4022Google Scholar

Copyright information

© Birkhäuser Boston 1993

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  • David M. Segal

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