Measurement of Cytolysin Hemolytic Activity

  • Pierre Henkart


Determine the lytic functional activity of cytolysin (perform) in granule and cell extracts. Red cell lysis is measured by optical absorbance of supernatant hemoglobin.


Polyethylene Glycol Optical Absorbance Ethanesulf Onic Acid Dilution Buffer Cytoplasmic Granule 


  1. Henkart PA, Millard PJ, Reynolds CW, Henkart MP (1984): Cytolytic activity of purified cytoplasmic granules from cytotoxic rat LGL tumors. J Exp Med 160: 75CrossRefGoogle Scholar
  2. Millard PJ, Henkart MP, Reynolds CW, Henkart PA (1984): Purification and properties of cytoplasmic granules from cytotoxic rat LGL tumors. J Immunol 132: 3197Google Scholar
  3. Yue CC, Reynolds CW, Henkart PA (1987): Inhibition of cytolysin activity in granules of large granular lymphocytes by lipids: Evidence for a membrane insertion mechanism of lysis. Mol Immunol 24: 647CrossRefGoogle Scholar

Copyright information

© Birkhäuser Boston 1993

Authors and Affiliations

  • Pierre Henkart

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