Granule Exocytosis Assay of CTL Activation

  • Michail V. Sitkovsky


Granule exocytosis assay for cytotoxic T lymphocyte (CTL) activation has become an important additional method of evaluating activating stimuli and of studying cell surface receptors and transmembrane signaling. It also provides the opportunity to study the effects of enhancing and inhibiting agents on the exocytic response by predictably manipulating the intensity of granule exocytosis which can be triggered by immobilized monoclonal antibody (mAb) or by the antigen (Ag)-bearing target cell. This assay is based on the ability of CTL to release cytoplasmic granules into the culture medium that contain the easily detectable enzyme serine esterase (Pasternack et al., 1986; Takayama and Sitkovsky, 1987). We describe here the granule exocytosis assay in response to immobilized anti-T-cell receptor (TCR) mAb and to Ag-bearing target cells. For more details the reader is directed to (Taffs and Sitkovsky (1991).


Granule Exocytosis Serine Esterase Immobilize Monoclonal Antibody Allotypic Determinant Optimum Stimulation Level 


  1. Leo O. Foo M. Sachs DH. Samelson LE. Bluestone JA (1987): Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Nail Acad Sci USA 84: 1374 - 1378CrossRefGoogle Scholar
  2. Pasternack M. Verret CR. Liu MA, Eisen HN (1986): Serine esterase in cytolytic T lymphocytes. Nature 322: 740 - 743CrossRefGoogle Scholar
  3. Taffs RE, Sitkovsky MV (1991): Granule enzyme exocytosis assay for CTL activation. In: Current Protocols in Immunology. Coligan J, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, eds. Unit 3. 16Google Scholar
  4. Takayama H, Sitkovsky MV (1987): Antigen receptor-regulated exocytosis in cytotoxic T lymphocytes. J Exp Med 166: 725 - 743CrossRefGoogle Scholar

Copyright information

© Birkhäuser Boston 1993

Authors and Affiliations

  • Michail V. Sitkovsky

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