Granule Exocytosis Assay of CTL Activation
Granule exocytosis assay for cytotoxic T lymphocyte (CTL) activation has become an important additional method of evaluating activating stimuli and of studying cell surface receptors and transmembrane signaling. It also provides the opportunity to study the effects of enhancing and inhibiting agents on the exocytic response by predictably manipulating the intensity of granule exocytosis which can be triggered by immobilized monoclonal antibody (mAb) or by the antigen (Ag)-bearing target cell. This assay is based on the ability of CTL to release cytoplasmic granules into the culture medium that contain the easily detectable enzyme serine esterase (Pasternack et al., 1986; Takayama and Sitkovsky, 1987). We describe here the granule exocytosis assay in response to immobilized anti-T-cell receptor (TCR) mAb and to Ag-bearing target cells. For more details the reader is directed to (Taffs and Sitkovsky (1991).
KeywordsGranule Exocytosis Serine Esterase Immobilize Monoclonal Antibody Allotypic Determinant Optimum Stimulation Level
- Taffs RE, Sitkovsky MV (1991): Granule enzyme exocytosis assay for CTL activation. In: Current Protocols in Immunology. Coligan J, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, eds. Unit 3. 16Google Scholar