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Protocol for Assaying CTL Activity Against Toxoplasma gondii

  • Ricardo T. Gazzinelli
  • Eric Denkers
  • Frances Hakim
  • Alan Sher

Abstract

Tachyzoites of the ts-4 strain of T. gondii are maintained by in vitro passage in human foreskin fibroblasts at 33°C. T. gondii soluble antigens (STAg) are prepared from parasites obtained by peritoneal lavage, 3 days after infection of Swiss Webster mice with 5–10 × 106 RH strain tachyzoites. Tachyzoites are concentrated by centrifugation (1500 × g, 10 min) and repeatedly sonicated until no intact parasites remain. After centrifugation at 10,000 × g for 30 min, the supernatant is collected, dialyzed against PBS, and stored at −80°C.

Keywords

Nonessential Amino Acid Sodium Pyruvate Peritoneal Lavage Soluble Antigen Human Foreskin Fibroblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Birkhäuser Boston 1993

Authors and Affiliations

  • Ricardo T. Gazzinelli
  • Eric Denkers
  • Frances Hakim
  • Alan Sher

There are no affiliations available

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