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Target Cell Detachment Assay

  • Scott I. Abrams
  • John H. Russell

Abstract

Conventional assays used for the measurement of cell-mediated cytotoxicity involve interactions between effector lymphocytes and antigen-bearing targets maintained and/or manipulated into a suspension culture. However, in vivo,immunologic reactions also involve T lymphocytes responding to targets that are bound to each other or to an extracellular matrix. To that end, we have developed an in vitro murine model, which more closely mimics such an in vivo situation, to explore the functional consequences that result from T cell-tissue or tumor interaction (Russell et al., 1988).

Keywords

Spontaneous Release Supernatant Fluid Conventional Assay Effector Lymphocyte Warm Culture Medium 
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References

  1. Abrams SI, McCulley DE, Meleedy-Rey P, Russell JH (1989): Cytotoxic T lymphocyte (CTL)-induced loss of target cell adhesion and lysis involve common and separate CTL signaling pathways. J Immunol 142: 1789–1796Google Scholar
  2. Abrams SI, Russell JH (1991): CD4+ T lymphocyte-induced target cell detachment. A model for T cell-mediated lytic and nonlytic inflammatory processes. J Immunol 146: 405–413Google Scholar
  3. Abrams SI, Russell JH (1991): CD4+ T lymphocyte-induced target cell detachment. A model for T cell-mediated lytic and nonlytic inflammatory processes. J Immunol 146: 405–413Google Scholar
  4. Russell JH, Musil L, McCulley DE (1988): Loss of adhesion. A novel and distinct effect of the cytotoxic T lymphocyte-target interaction. J Immunol 140: 427–432Google Scholar

Copyright information

© Birkhäuser Boston 1993

Authors and Affiliations

  • Scott I. Abrams
  • John H. Russell

There are no affiliations available

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