DNA Fragmentation and Cytolysis Assayed by 3H-Thymidine
The present note has two purposes: first, to recommend the use of 3H-thymidine over iododeoxyuridine-125 (125IUdR) for assays of DNA fragmentation; and second, to point out the advantages of using the same 3H-thymidine label to assay cytolysis (instead of using double label with 51Cr). We have recently published data in which both DNA fragmentation and lysis were quantitated with 3H-thymidine (Martz and Gamble, 1992).
KeywordsLiquid Scintillation Auger Electron Double Label Sensitive Cell Line Spleen Cell Proliferation
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