Abstract
The present note has two purposes: first, to recommend the use of 3H-thymidine over iododeoxyuridine-125 (125IUdR) for assays of DNA fragmentation; and second, to point out the advantages of using the same 3H-thymidine label to assay cytolysis (instead of using double label with 51Cr). We have recently published data in which both DNA fragmentation and lysis were quantitated with 3H-thymidine (Martz and Gamble, 1992).
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References
Duke RC, Cohen JJ (1988): The role of nuclear damage in lysis of target cells by cytotoxic T lymphocytes. In: Cvtolytic Lymphocytes and Complement: Effectors of the Immune System, Podack ER, ed. Boca Raton, FL: CRC Press, 2: 35–37
Duke RC, Cohen JJ (1992): Cell death and apoptosis. In: Current Protocols in Immunology,Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, eds. Brooklyn, NY: Greene Publishing Associates (in press)
Howell DM, Martz E (1987): The degree of CTL-induced DNA solubilization is not determined by the human vs. mouse origin of the target cell. J Immunol 138: 3695–3698
Howell DM, Martz E (1988): Evidence against damage to the nuclear envelope of the target cell in the induction of nuclear disintegration by cytolytic T lymphocytes. J Immunol 140: 689–692
Martz E, Gamble SR (1992): How do CTL control virus infections? Evidence for prelytic halt of Herpes simplex. Virol Immunol (in press)
Martz E, Howell DM (1989): CTL: Virus control cells first and cytolytic cells second? DNA fragmentation, apoptosis, and the prelytic halt hypothesis. Immunol Today 10: 79–86
Russell, JH (1983): Internal disintegration model of cytotoxic lymphocyte-induced target damage. Immunol Rev 72: 97–118
Stadecker MJ, Calderon J, Karnovsky ML, Unanue ER (1977): Synthesis and release of thymidine by macrophages. J Immunol 119: 1738–1743
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© 1993 Birkhäuser Boston
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Martz, E. (1993). DNA Fragmentation and Cytolysis Assayed by 3H-Thymidine. In: Sitkovsky, M.V., Henkart, P.A. (eds) Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods. Birkhäuser Boston. https://doi.org/10.1007/978-1-4684-6814-4_44
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DOI: https://doi.org/10.1007/978-1-4684-6814-4_44
Publisher Name: Birkhäuser Boston
Print ISBN: 978-1-4684-6816-8
Online ISBN: 978-1-4684-6814-4
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