Immunobiology and Molecular Characteristics of Peritoneal Exudate Cytotoxic T Lymphocytes (PEL), Their In Vivo IL-2-Dependent Blasts and IL-2-Independent Cytolytic Hybridomas

  • Gideon Berke
  • Dalia Rosen
  • Denise Ronen
  • Barbara Schick

Abstract

Major histocompatibility complex (MHC)-restricted cytolytic T lymphocytes (CTL) are generated in response to allogeneic tissues (normal and malignant), tumors (autologous and syngeneic), viruses, and certain bacteria and self-antigens. CTL can be derived directly from spleen or lymph nodes after immunization, but these cells often require a secondary in vitro stimulation (Cerottini and Brunner. 1974). Peritoneal exudate CTL (PEL), collected during or shortly after a primary intraperitoneal (i.p.) immunization of rats and mice with allogeneic or irradiated syngeneic tumors, usually yield a highly potent, specific population of CTL (Berke et al., 1972a, 1972b, 1972c; Fishelson and Berke, 1978; Schick and Berke, 1977; Berke and Schick, 1980). Generation of PEL can be augmented by, but does not require, a secondary stimulation in vivo or in vitro.

Keywords

Migration Chromium Lymphoma Leukemia Cysteine 

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Copyright information

© Birkhäuser Boston 1993

Authors and Affiliations

  • Gideon Berke
  • Dalia Rosen
  • Denise Ronen
  • Barbara Schick

There are no affiliations available

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