Quantitative Whole Body Autoradiographic Microimaging for Pharmaceutical Research

  • Prantika Som
  • Zvi H. Oster


Autoradiographic (ARG) microimaging is probably the highest spatial resolution method for imaging the distribution of radiolabeled compounds. This method can be used in sections of whole small animals such as mice, rats and even small monkeys, or in parts of animals, using the new large-block cryomicrotomes. Radiolabeled compounds and their metabolites have been studied in normal animals as well as in animal models of human disease. By sacrificing animals at various time intervals after the injection of the radiocompounds, the dimension of time can be introduced, thus generating data showing the temporo-spatial distribution and biokinetic pathways of compounds. This technique can be used to study the biodistribution of new compounds, the toxicity of substances, and the effects of pharmacological interventions of various active compounds (Som and Sacker, 1992; Ullberg, 1954; Berlin and Ullberg, 1963a; Berlin and Ullberg, 1963b; Cohen and Hood, 1969; Busch, 1977, Cross, 1977a; Cross, 1977b; Som et al., 1983; d’Argy et al., 1984; Yonekura et al., 1985).


Prussian Blue Fatty Acid Uptake Placental Transfer Hypertensive Heart Disease Cocaine Uptake 
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Copyright information

© Birkhäuser Boston 1993

Authors and Affiliations

  • Prantika Som
    • 1
  • Zvi H. Oster
    • 2
  1. 1.Medical DepartmentBrookhaven National LaboratoryUSA
  2. 2.Department of RadiologyState University of New York at Stony BrookUSA

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