Protection Against Oxidative Damage to CNS by α-Phenyl-tert-butyl Nitrone and Other Spin-Trapping Agents: A Novel Series of Nonlipid Free Radical Scavengers
Observations have been made in the last few years clearly indicating that ROS (reactive oxygen species which include oxygen free radicals, singlet oxygen, lipid hydroperoxides, and hydrogen peroxide) are etiologic agents in the mechanism of development of several pathologic conditions. Thus, recent research efforts have implicated ROS in the following: ischemia/reperfusion injury, arthritis, aging, carcinogenesis, hyperbaric oxygen toxicity, ionizing irradiation injury, ozone damage, damage caused by some xenobiotics, and the mechanism of action of some antitumor agents, etc. Progress has been made possible by new methodologies that more accurately quantitate the in vivo flux of oxygen free radicals. Two of these newer methodologies include salicylate trapping of hydroxyl free radical flux in vivo and the use of spin-trapping compounds to quantitate and characterize the free radicals being produced in vivo. Our laboratories have been active in the development and use of these new methodologies, and in the course of this work we have made the fascinating observation that certain spin-trapping agents are very effective in protecting brain from oxidative damage.
KeywordsElectron Paramagnetic Resonance Glutamine Synthetase Oxygen Free Radical Protein Oxidation Hydroxyl Free Radical
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