The Use of Protoplast Technology in Tissue Culture of Trees

  • John H. Dodds


Isolated plant protoplasts can be described as naked plant cells. Protoplasts are plant cells where the cell wall has been removed either by a mechanical or enzymic method. The original way of preparing protoplasts was by plasmolysis of the tissue followed by mechanical abrasion. The cell wall would then allow release of the plasmolysed protoplast. The mechanical method of protoplast isolation has the disadvantages that it is very tedious and time consuming and more importantly only very small numbers of protoplasts can be isolated. One of the advantages, however, of mechanical isolation is that there are no enzyme or enzyme impurities present to attack and weaken the plasma membrane of the isolated protoplast. In the 1960s techniques were developed for the isolation of protoplasts from leaf tissue using the enzymes cellulase and pectinase: these enzymic techniques offer a quick and efficient method for the isolation of very large numbers of protoplasts.


Protoplast Fusion Crown Gall Plant Protoplast Foreign Genetic Material High Heavy Metal Content 
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© John H. Dodds 1983

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  • John H. Dodds

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