The Influence of Physical Factors on Xylem Differentiation In Vitro

  • Lorin W. Roberts


Temperature, water, light, gas composition, mechanical stress and acidity are the most important physical factors in the modification of tracheary element differentiation in cultured plant tissues. The significance of some of these factors in the initiation of cytodifferentiation was reviewed by Roberts (1976). Investigations on the possible effects of physical factors on tracheary element formation in vitro can be subdivided into three categories: cellular, callus and aggregate cultures in suspension, and regenerated plantlets. There has been little or no research conducted on single cells; most studies have examined the role of physical variables in callus cultures. Research in the third and most important category, i.e. the influence of physical factors on tracheary element production in regenerated plantlets, is still in its infancy. The leader in the latter field has been Murashige (1974), who devised the optimum cultural conditions for many species of plantlets during stage III, which involves the transition between in vitro and in vivo growth. Unfortunately, these studies on environmental factors have not been concerned with the optimal physical conditions for secondary differentiation of vascular tissues during the early growth period of these young plantlets.


Plant Tissue Culture Compression Wood Tension Wood Tracheary Element Secondary Xylem 
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  1. Adams, D.O. and Yang, S.F. (1981) ‘Ethylene, the gaseous plant hormone: mechanism and regulation of biosynthesis’, Trends in Biochem. Sci., 6, 161–3CrossRefGoogle Scholar
  2. Aharoni, N. and Lieberman, M. (1979) ‘Ethylene as a regulator of senescence in tobacco leaf discs’, Plant Physiol., 64, 801–4CrossRefGoogle Scholar
  3. Bassi, P.K. and Spencer, M.S. (1982) ‘Effect of carbon dioxide and light on ethylene production in intact sunflower plants’, Plant Physiol., 69, 1222–5CrossRefGoogle Scholar
  4. Beyer, E.M., Jr. (1979) ‘Effect of silver ion, carbon dioxide, and oxygen on ethylene action and metabolism’, Plant Physiol., 63, 169–73CrossRefGoogle Scholar
  5. Bornman, C.H. and Huber, W. (1979) ‘Nicotiana tabacum callus studies. 9. De- velopment in stressed explants’, Biochemie Physiol. Pfanzen, 174, 345–56Google Scholar
  6. Bradford, K.J. and Yang, S.F. (1980) ‘Xylem transport of 1-aminocyclopropane1-carboxylic acid, an ethylene precursor, in waterlogged tomato plants’, Plant Physiol., 65, 322–6CrossRefGoogle Scholar
  7. Bradley, M.V. and Dahmen, W.J. (1971) ‘Cytohistological effects of ethylene, 2, 4-D, kinetin and carbon dioxide on peach mesocarp callus cultured in vitro’, Phytomorphology, 21, 154–64Google Scholar
  8. Brown, C.L. (1964) ‘The influence of external pressure on the differentiation of cells and tissues cultured in vitro’ in M.H. Zimmermann (ed.), The Formation of Wood in Forest Trees, Academic Press, New York, pp. 389–404Google Scholar
  9. Brown, C.L. and Sax, K. (1962) ‘The influence of pressure on the differentiation of secondary tissues’, Am. J. Bot., 49, 683–91CrossRefGoogle Scholar
  10. Brown, K.M. and Leopold, A.C. (1973) ‘Ethylene and the regulation of growth in pine’, Can. J. For. Res., 3, 143–5CrossRefGoogle Scholar
  11. Chafe, S.C. and Durzan, D.J. (1973) ‘Tannin inclusions in cell suspension cultures of white spruce’, Planta, 113, 251–62CrossRefGoogle Scholar
  12. Datta, S.K., Chakrabarti, K. and Datta, P.C. (1975) ‘In vitro effect of acidity levels on xylem differentiation in Plumeria’, Current Sci., 44, 814–16Google Scholar
  13. Davidson, A.W. and Yeoman, M.M. (1974) ‘A phytochrome-mediated sequence of reactions regulating cell division in developing callus cultures’, Ann. Bot., 38, 545–54Google Scholar
  14. Denne, M.B. (1971) ‘Temperature and tracheid development in Pinus sylvestris seedlings’, J. Exp. Bot., 22, 362–70CrossRefGoogle Scholar
  15. Denne, M.B. and Dodd, R.S. (1981) ‘The environmental control of xylem differentiation’ in J.R. Barnett (ed.), Xylem Cell Development, Castlehouse Publications Ltd., Tunbridge Wells, U.K., pp. 236–55Google Scholar
  16. Dodds, J.H. and Roberts, L.W. (1982) Experiments in Plant Tissue Culture, University Press, CambridgeGoogle Scholar
  17. Doley, D. and Leyton, L. (1970) ‘Effects of growth regulating substances and water potential on the development of wound callus in Fraxinus’, New Phytol., 69, 87–102CrossRefGoogle Scholar
  18. Dudley, K. and Northcote, D.H. (1979) ‘Regulation of induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris’, Planta, 146, 433–40Google Scholar
  19. Durzan, D.J., Chafe, S.C. and Lopushanski, S.M. (1973) ‘Effects of environmental changes on sugars, tannins, and organized growth in cell suspension cultures of white spruce’, Planta, 133, 241–9CrossRefGoogle Scholar
  20. Esau, K. (1977) Anatomy of Seed Plants, 2nd edn., J. Wiley and Sons, New York.Google Scholar
  21. Gautheret, R.J. (1961) ‘Action de la lumière et de la température sur la néoformation de racines par des tissus de Topinambour cultivés in vitro’, Compte. Rendu. Acad. Sci., 250, 2791–6Google Scholar
  22. Gautheret, R.J. (1969) ‘Investigations on the root formation in the tissues of Helianthus tuberosus cultured in vitro’, Am. J. Bot., 56, 702–17Google Scholar
  23. Grisebach, H. and Hahlbrock, K. (1974) ‘Enzymology and regulation of flavanoid and lignin biosynthesis in plants and plant cell suspension cultures’ in V.C. Runeckles and E.E. Conn (eds.), Metabolism and Regulation of Secondary Plant Products, Academic Press, New York, pp. 22–52Google Scholar
  24. Grout, B.W.W. and Aston, M.J. (1977) ‘Transplanting of cauliflower plants regenerated from meristem culture. I. Water loss and water transfer related to changes in leaf wax and to xylem regeneration’, Hort. Res., 17, 1–7Google Scholar
  25. Haddon, L.E. and Northcote, D.H. (1975) ‘Quantitative measurement 6f bean callus differentiation’, J. Cell Sci., 17, 11–26Google Scholar
  26. Haddon, L.E. and Northcote, D.H. (1976) ‘Correlation of the induction of various enzymes concerned with phenylpropanoid and lignin synthesis during differentiation of bean callus (Phaseolus vulgaris L.)’, Planta, 128, 255–62CrossRefGoogle Scholar
  27. Hahlbrock, K. and Grisebach, H. (1979) ‘Enzymic controls in the biosynthesis of lignin and flavonoids’, Ann. Rev. Plant Physiol., 30, 105–30CrossRefGoogle Scholar
  28. Hunt, E.R., Jr. and Jaffe, M.J. (1980) ‘Thigmomorphogenesis: the interaction of wind and temperature in the field on the growth of Phaseolus vulgaris L.’, Ann. Bot., 45, 665–72Google Scholar
  29. Jacobs, M.R. (1954) ‘The effect of wind sway on the form and development of Pinus radiata D. Don’, Aust. J. Bot., 2, 35–51CrossRefGoogle Scholar
  30. Jacquoit, C. (1947) ‘Effet inhibiteur des tannins sur le développement des cultures in vitro du cambium de certaines arbres forestiers’, Compte. Rendu. Acad. Sci., 225, 434–6Google Scholar
  31. Jaffe, M.J. (1980) ‘Morphogenetic responses of plants to mechanical stimuli or stress’, BioSci., 30, 239–43CrossRefGoogle Scholar
  32. Jaffe, M.J. and Biro, R. (1979) ‘Thigmomorphogenesis: the effect of mechanical perturbation on the growth of plants, with special reference to anatomical changes, the role of ethylene, and interaction with other environmental stresses’ in H. Mussell and R.C. Staples (eds.), Stress Physiology in Crop Plants, J. Wiley and Sons, New York, pp. 25–69Google Scholar
  33. James, D.J. and Davidson, A.W. (1977) ‘Phytochrome control of phenylalanine ammonia-lyase levels and the regulation of cell division in artichoke callus cultures’, Ann. Bot., 41, 873–7Google Scholar
  34. Jane, F.W. (1970) The Structure of Wood, 2nd edn, Adam and Charles Black, LondonGoogle Scholar
  35. Johnson, D.C. and Roberts, L.W. (1978) ‘Water stress influences xylogenesis in cultured explants of Lactuca’, Phytomorphology, 28, 207–9Google Scholar
  36. Kessell, R.H.J. and Carr, A.H. (1972) ‘The effect of dissolved oxygen concentration on growth and differentiation of carrot (Daucus carota) tissue’, J. Exp. Bot., 23, 996–1007CrossRefGoogle Scholar
  37. Kirkham, M.B., Gardner, W.R. and Gerloff, G.C. (1972) ‘Regulation of cell division and cell enlargement by turgor pressure’, Plant Physiol., 49, 961–2CrossRefGoogle Scholar
  38. Kleiber, H. and Mohr, H. (1967) ‘Vom Einfluss des Phytochroms auf die Xylemdifferzierung im Hypokotyl des Senfkimlings (Sinapis alba L.)’, Planta, 76, 85–92CrossRefGoogle Scholar
  39. Lamb, C.J. (1979) ‘Regulation of enzyme levels in phenylpropanoid biosynthesis: characterization of the modulation by light and pathway intermediates’, Arch. Biochem. Biophys., 192, 311–17CrossRefGoogle Scholar
  40. Larson, P.R. (1965) ‘Stem form of young Larix as influenced by wind and pruning’, Forest Sci., 11, 412–24Google Scholar
  41. Leopold, A.C. (1972) ‘Ethylene as a plant hormone’ in H. Kaldewey and Y. Vardar (eds.), Hormonal Regulation in Plant Growth and Development, Verlag Chemie, Weinheim, pp. 245–62Google Scholar
  42. Lieberman, M. (1979) ‘Biosynthesis and action of ethylene’, Ann. Rev. Plant Physiol., 30, 533–91CrossRefGoogle Scholar
  43. Lintilhac, P.M. and Vesecky, T.B. (1981) ‘Mechanical stress and cell wall orientation in plants. II. The application of controlled directional stress to growing plants; with a discussion on the nature of the wound reaction’, Am. J. Bot., 68, 1222–30CrossRefGoogle Scholar
  44. McDaniel, R.G. (1982) ‘The physiology of temperature effects on plants’ in M.N. Christiansen and C.F. Lewis (eds.), Breeding Plants for Less Favorable Environments, J. Wiley and Sons, New York, pp. 13–45Google Scholar
  45. Martin, S.M. (1980) ‘Environmental factors. B. Temperature, aeration, and pH’ in E.J. Staba (ed.), Plant Tissue Culture as a Source of Biochemicals, CRC Press, Boca Raton, Florida, pp. 143–8Google Scholar
  46. Miller, A.R. and Roberts, L.W. (1982) ‘Regulation of tracheary element differentiation by exogenous L-methionine in callus of soybean cultivars’, Ann. Bot. (in press)Google Scholar
  47. Mitchell, C.A. (1977) ‘Influences of mechanical stress on auxin-stimulated growth of excised pea stem sections’, Physiol. Plant, 41, 129–34CrossRefGoogle Scholar
  48. Mizuno, K. and Komamine, A. (1978) ‘Isolation and identification of substances inducing formation of tracheary elements in cultured carrot-root slices’, Planta, 138, 59–62CrossRefGoogle Scholar
  49. Mizuno, K., Komamine, A. and Shimokoriyama, M. (1971) ‘Vessel element formation in cultured carrot-root phloem’, Plant Cell Physio1., 12, 823–30Google Scholar
  50. Murashige, T. (1974) ‘Plant propagation through tissue cultures’, Ann. Rev. Plant Physio1., 25, 135–66CrossRefGoogle Scholar
  51. Murashige, T. and Skoog, F. (1962) ‘A revised medium for rapid growth and bioassays with tobacco tissue cultures’, Physiol. Plant., 15, 473–97CrossRefGoogle Scholar
  52. Naik, G.G. (1965) ‘Studies on the effects of temperature on the growth of plant tissue cultures’, M.Sc. Thesis, University of Edinburgh, Scotland.Google Scholar
  53. Nelson, N.D. and Hillis, W.E. (1978) ‘Ethylene and tension wood formation in Eucalyptus gomphocephala’, Wood Sci. Tech., 12, 309–15Google Scholar
  54. Parsons, L.R. (1982) ‘Plant responses to water stress’ in M.N. Christiansen and C.F. Lewis (eds.), Breeding Plants for Less Favorable Environments, J. Wiley and Sons, New York, pp. 175–92Google Scholar
  55. Phillips, R. and Dodds, J.H. (1977) ‘Rapid differentiation of tracheary elements in cultured explants of Jerusalem artichoke’, Planta, 135, 207–12CrossRefGoogle Scholar
  56. Quirk, J.T., Smith, D.M. and Freese, F. (1975) ‘Effect of mechanical stress on growth and anatomical structure of red pine (Pinus resinosa Ait.): torque stress’, Can. J. For. Res., 5, 691–9CrossRefGoogle Scholar
  57. Reinhold, L., Sachs, T. and Vislovska, L. (1972) ‘The role of auxin in thigmotropism’ in D.J. Carr (ed.), Plant Growth Substances, 1970, Springer-Verlag, Berlin, pp. 731–7CrossRefGoogle Scholar
  58. Rier, J.P., Jr. (1976) ‘Ozone and vascular tissue differentiation in plants’, U.S. Environmental Protection Agency, Washington, D.C. (EPA–600/3–76–068)Google Scholar
  59. Roberts, L.W. (1976) Cytodifferentiation in Plants: Xylogenesis as a Model System, University Press, CambridgeGoogle Scholar
  60. Roberts, L.W. and Baba, S. (1968) ‘Effect of proline on wound vessel member formation’, Plant Cell Physiol., 9, 353–60Google Scholar
  61. Roberts, L.W. and Baba, S. (1978) ‘Exogenous methionine as a nutrient supplement for the induction of xylogenesis in lettuce pith explants’, Ann. Bot., 42, 375–9Google Scholar
  62. Roberts, L.W., Baba, S., Shiraishi, T. and Miller, A.R. (1982) ‘Progress in cytodifferentiation under in vitro conditions’, Proc. V Internat. Congress of Plant Tissue and Cell Culture, IAPTC, TokyoGoogle Scholar
  63. Robitaille, H.A. (1975) ‘Stress ethylene production in apple shoots’, J. Am. Soc. Hort. Sci., 100, 5 24–7Google Scholar
  64. Robitaille, H.A. and Leopold, A.C. (1974) ‘Ethylene and the regulation of apple stem growth under stress’, Physiol. Plant, 32, 301–4CrossRefGoogle Scholar
  65. Rubery, P.H. and Fosket, D.E. (1969) ‘Changes in phenylalanine ammonia-lyase during xylem differentiation in Coleus and soybean’, Planta, 87, 54–62CrossRefGoogle Scholar
  66. Savidge, R.A. and Wareing, P.F. (1981) ‘Plant-growth regulators and the differentiation of vascular elements’ in J.R. Barnett (ed.), Xylem Cell Development, Castlehouse Publications Ltd., Tunbridge Wells, U.K., pp. 192–235Google Scholar
  67. Seibert, M. and Kadkade, P.G. (1980) ‘Environmental factors. A. Light’ in E.J. Staba (ed.), Plant Tissue Culture as a Source of Biochemicals, CRC Press, Boca Raton, Florida, pp. 123–41Google Scholar
  68. Shininger, T.L. (1979a) `Xylem and nonxylem cell formation in cytokininstimulated root tissue. Quantitative analysis of temperature effects’, Proc. Nat. Acad. Sci. (USA), 76, 1921–3Google Scholar
  69. Shininger, T.L. (1979b) The control of vascular development’, Ann. Rev. Plant Physiol., 30, 313–37CrossRefGoogle Scholar
  70. Syóno, K. and Furuya, T. (1971) ‘Effects of temperature on the cytokinin requirement of tobacco calluses’, Plant Cell Physiol., 12, 61–71Google Scholar
  71. Timell, T.E. (1973) ‘Ultrastructure of the dormant and active cambial zones and the dormant phloem associated with formation of normal and compression woods in Picea abies (L.) Karst’, Pub. 96, Office of Public Service and Continuing Education, State University of New York College of Environmental Science and Forestry, SyracuseGoogle Scholar
  72. Wellmann, E. and Schoffer, P. (1975) ‘Phytochrome-mediated de novo synthesis of phenylalanine ammonia-lyase in cell suspension cultures of parsley’, Plant Physiol., 55, 822–8CrossRefGoogle Scholar
  73. Windholz, M. (ed.) (1976) The Merck Index. An Encyclopedia of Chemicals and Drugs, 9th edn, Merck, Rahway, New Jersey, USAGoogle Scholar
  74. Wright, K. and Northcote, D.H. (1973) ‘Differences in ploidy and degree of intercellular contact in differentiating and non-differentiating sycamore calluses’, J. Cell Sci., 12, 37–53Google Scholar
  75. Yamaguchi, K., Itoh, T. and Shimaji, K. (1980) ‘Compression wood induced by 1-N-naphthylphthalamic acid (NPA), an IAA transport inhibitor’, Wood Sci. Tech., 14, 181–5Google Scholar
  76. Yeoman, M.M. and Brown, R. (1971) ‘Effects of mechanical stress on the plane of cell division in developing callus cultures’, Ann. Bot., 35, 1101–12Google Scholar
  77. Yeoman, M.M. and Davidson, A.W. (1971) `Effect of light on cell division in developing callus cultures’, Ann. Bot., 35, 1085–1100Google Scholar

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© John H. Dodds 1983

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  • Lorin W. Roberts

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