Tissue Culture of Hardwoods

  • John H. Dodds


The previous chapter has dealt with tissue culture of softwoods (conifers). This chapter will deal with hardwoods, but because of the diversity of this group many examples will be omitted or dealt with in subsequent chapters. As with the gymnosperms the hardwood trees cover an enormous geographical area and a wide range of climatic conditions.


Apical Meristem Shoot Apex Callus Culture Cambial Zone Hardwood Tree 
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  1. Abbott, A.J. and Whiteley, E. (1974) In vitro regeneration and multiplication of apple tissues’ in 3rd International Conference of Plant Tissue Culture, Abst. 263 (Leicester 1974)Google Scholar
  2. Altman, A. and Goren, R. (1971) Promotion of callus formation by ABA in Citrus bud cultures’, Plant Physiol., 47, 844–6CrossRefGoogle Scholar
  3. Berbee, F.M., Berbee, J.G. and Hildebrandt, A.C. (1972) Induction of callus and trees from stem tip cultures of a hybrid Popular’, In Vitro, 7, 269–73Google Scholar
  4. Bitters, W.P., Murashige, T., Rangan, T.S. and Nauer, E. (1972) Investigations on establishing virus-free Citrus plants through tissue culture’ in W.C. Price (ed.), Sth International Congress of the International Organ of Citrus Virology, Gainesville, Univ. of Florida, pp. 267–71Google Scholar
  5. Bonga, J.M. (1977) Applications of tissue culture in forestry’ in J. Reinert and Y.P.S. Bajaj (eds.), Plant Cell and Organ Culture, Springer-Verlag, Berlin, pp. 93–108Google Scholar
  6. Carlisle, A. and Teich, A.H. (1971) The costs and benefits of tree improvement programs’, Can. For. Serv. Pub., 1302 Google Scholar
  7. Chalupa, V. (1974) Control of root and shoot formation and production of trees from Poplar callus’, Biol. Plant., 16, 316–20CrossRefGoogle Scholar
  8. Cutter, E.G. (1965) Recent experimental studies of the shoot apex and shoot morphogenesis’, Bot. Rev., 31, 7–113CrossRefGoogle Scholar
  9. D’amato, F. (1965) Endopolyploidy as a factor in plant tissue development’ in P. White and A.R. Grove (eds.), Proceedings of the International Conference on Plant Tissue Culture, Berkley 1965, pp. 449–62Google Scholar
  10. D’amato, F. (1978) Endopolyploidy as a factor in development’, Proceedings of 4th International Plant Tissue Culture Congress, Calgary, Canada 1978Google Scholar
  11. David, A. (1982) In vitro propagation of gymnosperms’ in J.M. Bonga and D.J. Durzan (eds.), Tissue Culture in Forestry, Junk, Netherlands, pp. 72–108Google Scholar
  12. David, A. and David, H. (1979) Isolation and callus formation from cotyledon protoplasts of pine (Pinus pineaster)’, Z. Pflanzenphysiol., 94, 173–7Google Scholar
  13. Durzan, D.J. and Campbell, R.A. (1974a) Prospects for the mass production of improved stock of forest trees by cell and tissue culture’, Canadian Journal of Forestry Research, 4, 151–74CrossRefGoogle Scholar
  14. Durzan, D.J. and Campbell, R.A. (1974b) Prospects for the introduction of traits in forest trees by cell and tissue culture’, N.Z. J. Forestry Sci., 4, 261–6Google Scholar
  15. Gautheret, R.J. (1934) Culture du tissu cambial’, Compte. Rendu. Acad. Sci., 198, 2195–6Google Scholar
  16. Gautheret, R.J. (1937) Nouvelles recherches sur la culture du tissu cambial’, Compte. Rendu. Acad. Sci., 205, 572–4Google Scholar
  17. Gautheret, R.J. (1948) Sur la culture indéfinie des tissus de Salix caprea’, Compte. Rendu. Soc. Biol., 142, 807–8Google Scholar
  18. Grashan, J.L. and Harvey, A.E. (1970) Preparative techniques and tissue selection criteria for in vitro culture of healthy and rust infected conifer tissues’, U.S. Dept. Agr. Forest Ser. Res. Pap. Int., 82, 1970Google Scholar
  19. Hutchinson, O. and Yahyaoglu, Z. (1974) Das frühe Blühen von aus Kalluskulturen herangezogene Pflänzchen bei der Birko (Betula pendula)’, Silvae Genet., 23, 1–3Google Scholar
  20. Jacquiot, C. (1950) Sur la culture in vitro de tissue cambial de Châtaignier (Castanea vesca)’, Compte. Rendu. Acad. Sci., 231, 1080–1Google Scholar
  21. Jacquiot, C. (1951) Action du meso-inositol et de l’adenine sur la formation de bourgeons par le tissu cambial d’Ulmus campestris cultive in vitro’, Compte. Rendu. Acad. Sci., 233, 815–17Google Scholar
  22. Jacquiot, C. (1966) Plant tissues and excised organ cultures and their significance in forest research’, J. Inst. Wood Sci., 16, 22–34Google Scholar
  23. Jones, L.H., Barfield, D., Barret, J., Flook, A., Pollock, K. and Robinson, P. (1982) Cytology of oil palm cultures and regenerated plants’, Proceedings 5th International Plant Tissue Culture Congress, Tokyo 1982 Google Scholar
  24. Kartha, K.K. and Gamborg, O.L. (1975) Elimination of cassava mosaic disease by meristem culture’, Phytopathology, 65, 826–8CrossRefGoogle Scholar
  25. Konar, R.N. and Nagmani, R. (1974) Tissue culture as a method for vegetative propagation of forest trees’, N.Z. J. Forestry Sci., 4, 279–90Google Scholar
  26. Libby, W.J., Stettler, R.F. and Seitz, F.W. (1969) Forest genetics and forest tree breeding’, Ann. Rev. Gen., 3, 469–94CrossRefGoogle Scholar
  27. Morel, G. (1948) Recherches sur la culture associée de parasites obligatoires et de tissus végétaux’, Ann. Epiphyties, 14, 123–234Google Scholar
  28. Mott, R.L. (1978) Trees’ in B.V. Conger (ed.), Cloning Agricultural Plants via In vitro techniques, CRC Press, FloridaGoogle Scholar
  29. Mott, R.L. and Amerson, H. (1981) A tissue culture process for the clonal production of Loblolly Pine plantlets’, N. Carolina Agr. Serv. Tech. Bull. No. 271 Google Scholar
  30. Murashige, T. (1974a) Plant propagation through tissue cultures’, Ann. Rev. Plant. Physiol., 25, 135–65CrossRefGoogle Scholar
  31. Murashige, T. (1974b) Plant cell and organ culture methods in the establishment of pathogen free stock’, No. 2. A.W. Dimock Lectures, Dept. of Pathology, Cornell University, Ithaca N. Y.Google Scholar
  32. Nitsch, J.P. (1963) Naturally-occurring growth substances in relation to plant tissue culture’ in P. Maheshwari and N.S. Rangaswamy (eds.), Plant Tissue and Organ Culture: A symposium, International Society of Plant Morphologists, Delhi, India, 1963, pp. 144–57Google Scholar
  33. Phillips, D.A. and Wareing, P.F.W. (1982) The control of growth and differentiation in plants ( 3rd edition ), Pergamon Press, OxfordGoogle Scholar
  34. Savidge, R.A. (1982) Hormonal factors controlling differentiation’, Histochem. J. (in press)Google Scholar
  35. Savidge, R.A. and Wareing, P.F.W. (1981) Plant growth regulators and the differentiation of vascular elements’ in J.R. Barnett (ed.), Xylem Cell Development, Castle House, London, pp. 192–236Google Scholar
  36. Skoog, F. and Miller, C.O. (1957) Chemical regulation of growth and organ formation in plant tissues cultivated in vitro’ in Biological Action of Growth Substances, 11th Symposium of Society for Experimental Biology, pp. 118–31Google Scholar
  37. Smith, R.H. and Murashige, T. (1970) ’In vitro development of the isolated shoot apical meristem of angiosperms’, Am. J. Bot.,57, 562–8Google Scholar
  38. Sugawara, Y. and Sakai, A. (1974) Survival of suspension cultured sycamore cells cooled to the temperature of liquid nitrogen’, Plant PhysioL, 54, 722–4CrossRefGoogle Scholar
  39. Sussex, I.M. and Clutter, M.E. (1959) Seasonal growth periodicity of tissue explants from woody perennial plants in vitro’, Science, 129, 836–7CrossRefGoogle Scholar
  40. Thomas, E. (1981) ‘Plant regeneration from shoot culture derived protoplasts of tetraploid potato (Solanum tuberosum)’, Plant Sci. Lett., 23, 81–8Google Scholar
  41. Thulin, I.J. and Faulds, T. (1968) ‘The use of cuttings in the breeding and afforestation of Pynus radiata’, N.Z. J. Forestry, 13, 66–77Google Scholar
  42. Wilkins, C.P., Bengochea, T. and Dodds, J.H. (1982) ‘The use of in vitro methods for plant genetic conservation’, Outlook on Agriculture, 11, 67–73Google Scholar
  43. Wilkins, C.P. and Dodds, J.H. (1982a) ‘Effects of various growth regulators on in vitro growth of clony shoot tips’, Plant Growth Reg. (in press)Google Scholar
  44. Wilkins, C.P. and Dodds, J.H. (1982b) ‘Effect of physical support on rooting of Prunus and Pynus shoots in vitro,(in preparation)Google Scholar
  45. Wilkins, C.P. and Dodds, J.H. (1983) ‘The application of tissue culture techniques to problems of plant genetic conservation’, Science Prog. (in press)Google Scholar
  46. Winton, L.L. (1970) ‘Shoot and tree production from aspen tissue cultures’, Am. J. Bot., 57, 904–9CrossRefGoogle Scholar
  47. Winton, L.L. (1971) ‘Tissue culture propagation of European aspen’, Forest ScL, 17, 48–50 Google Scholar
  48. Winton, L.L. (1974a) ‘Addendum to the bibliography of tree callus cultures’, General Physiology Notes 19, Institute of Paper Chemistry, Wisconsin, USAGoogle Scholar
  49. Winton, L.L. (1974b) The use of callus, cell and protoplast cultures for tree improvement’, U.S.-ROC Coop Sci. Prog., Taipei Google Scholar
  50. Winton, L.L. and Stettler, R.F. (1974) ‘Utilization of haploids in tree breeding’ in K.J. Kasha (ed.), Haploids in Higher Plants: Advances and Potential, Guelph, New York, pp. 259–73Google Scholar
  51. Withers, L.A. (1980) ‘Tissue culture storage for genetic conservation’, International Board for Plant Genetic Resources/Food and Agriculture Organisation of United Nations, RomaGoogle Scholar

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© John H. Dodds 1983

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  • John H. Dodds

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