Gene Function pp 329-370 | Cite as

Investigation of Gene Structure and Function

  • Robert E. Glass


An understanding of the principles underlying cellular growth, of the interplay of regulatory circuits with physiological factors, and of the myriad processes that involve gene expression, relies ultimately on a knowledge of chromosome structure. There is a wide variation in the complexity of prokaryotic genomes. Nevertheless, each genetic element consists, in the simplest terms, of a series of genes (with their associated control regions), perhaps interspersed with non-coding — ‘silent’ — stretches: and, at the chemical level, of a macromolecule of nucleic acid.


Bacterial Sequence Transduce Phage Permissive Host Phage Capsid Polycistronic Operon 
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Genetic Analysis of Bacteria and their Viruses

  1. Hayes, W. (1968) The Genetics of Bacteria and their Viruses, 2nd edn ( Blackwell, Oxford).Google Scholar
  2. Bachmann, B.J. and Low, K.B. (1980) ‘Linkage map of Escherichia coli K-12, edition 6’, Microbiol. Rev., 44, 1–56.Google Scholar
  3. Beckwith, J. (1978) lac: ‘the genetic system’, in J.H. Miller and W.S. Reznikoff(eds.), The Operon ( Cold Spring Harbor Laboratory, New York ), pp. 11–30.Google Scholar
  4. Beckwith, J. and Rossow, P. (1974) ‘Analysis of genetic regulatory mechanisms’,A nn. Rev. Genet., 8,1–13.Google Scholar
  5. Clowes, R.C. (1960) ‘Fine genetic structure as revealed by transduction’, Symp. Soc. Gen. Microbiol., 10, 92–114.Google Scholar
  6. Franklin, N.C. (1978) ‘Genetic fusions for operon analysis’, Ann. Rev. Genet, 12, 193–221.CrossRefGoogle Scholar
  7. Kleckner, N., Roth, J. and Botstein, D. (1977) ‘Genetic engineering in vivo using translocatable drug-resistance elements: New methods in bacterial genetics’, J. MoL Biot, 116, 125–159.CrossRefGoogle Scholar
  8. Lederberg, E.M. (1960) ‘Genetic and functional aspects of galactose metabolism in Escherichia coli K12’, Symp. Soc. Gen. MicrobioL, 10, 115–131.Google Scholar
  9. Miller, J.H. (1972) Experiments in Molecular Genetics ( Cold Spring Harbor Laboratory, New York).Google Scholar
  10. Ullman, A. and Perrin, D. (1970) ‘Complementation in ß-galactosidase’, in J.R. Beckwith and D. Zipser (eds.), The Lactose Operon ( Cold Spring Harbor Laboratory, New York ), pp. 143–172.Google Scholar
  11. Yanofsky, C. (1967) ‘Gene structure and protein structure’, Sct Am., 216, 80–94.CrossRefGoogle Scholar
  12. Yanofsky, C. (1971) ‘Tryptophan biosynthesis in E. coli: Genetic determination of proteins involved’, J. Am. Med. Assoc., 218, 1026–1035.CrossRefGoogle Scholar
  13. Benzer, S. (1962) ‘The fine structure of the gene’, Sci. Am., 206, 70–84.Google Scholar
  14. Campbell, A. (1971) ‘Genetic structure’, in A.D. Hershey (ed.), The Bacteriophage Lambda ( Cold Spring Harbor Laboratory, New York ), pp. 13–44.Google Scholar
  15. Edgar, R.S., and Epstein, R.H. (1965) ‘The genetics of a bacterial virus’, Sci Am., 212, 70–78.CrossRefGoogle Scholar

Monitoring Gene Function

  1. Coakley, W.T., Bater, A.J. and Lloyd, D. (1977) ‘Disruption of microorganisms’, Adv. Microb. Physiol., 16, 279–341.CrossRefGoogle Scholar
  2. Cooper, T.G. (1977) The Tools of Biochemistry ( Wiley, New York )Google Scholar
  3. Gaál, Ö, Medgyesi, G.A. and Vereczkey, L. (1980) Electrophoresis in the Separation of Biological Macromolecules ( Wiley, Chichester).Google Scholar
  4. Kennell, D.E. (1971) ‘Principles and practices of nucleic acid hybridisation’,Prog. NucL Acid Res. Molec. BioL, 11,259–301.Google Scholar

Physical Analysis of Gene Structure

  1. Brown, D. and Stern, R. (1974) ‘Methods of gene isolation’, Ann. Rev. Biochem., 43, 667–693.CrossRefGoogle Scholar
  2. Davidson, N., Deonier, R.C., Hu, S. and Ohtsubo, E. (1975) ‘Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli, X: Deoxyribonucleic acid sequence organisation of F and F-primes, and their sequences involved in Hfr formation’, in D. Schlessinger (ed.), Microbiology 1974 ( American Society for Microbiology, Washington ), pp. 56–65.Google Scholar
  3. Fiandt, M., Hradecna, Z., Lozeron, H.A. and Szybalski, W. (1971) ‘Electron micrographic mapping of deletions, insertion and homologies in the DNAs of coliphage lambda and phi 80’, in A.D. Hershey (ed.), The Bacteriophage Lambda ( Cold Spring Harbor Laboratory, New York ), pp. 329–354.Google Scholar
  4. Fisher, H.W. and Williams, R.C. (1979) ‘Electron microscopic visualisation of nucleic acids and of their complexes with proteins’, Ann. Rev. Biochem., 48, 649–679.CrossRefGoogle Scholar
  5. Air, G.M. (1979) ‘DNA sequencing of viral genomes’, in H. Fraenkel-Conrat and R.R. Wagner (eds.), Comprehensive Virology, 13: Structure and Assembly ( Plenum Press, New York ), pp. 205–292.Google Scholar
  6. Arber, W. (1974) ‘DNA modification and restriction’,Prog. NucL Acid Res. Molec. BioL, 14, 1–37. Arber, W. (1979) ‘Promotion and limitation of genetic exchange’, Science, 205, 361–365.CrossRefGoogle Scholar
  7. Benzinger, R. (1978) ‘Transfection of Enterobacteriaceae and its applications’, Microbiot, Rev., 42, 194–236.Google Scholar
  8. Brammar, W.J. (1977) ‘The construction in vitro and exploitation of transducing derivatives of bacteriophage X’, Biochem. Soc. Trans., 5, 1633–1652.Google Scholar
  9. Collins, J. (1978) ‘Gene cloning with small plasmids’, Curr. TopicsMicrobioL Immunol., 78, 122–173.Google Scholar
  10. Curtiss, R. (1976) ‘Genetic manipulation of microorganisms: Potential benefits and biohazards’, Ann. Rev. MicrobioL, 30, 507–533.CrossRefGoogle Scholar
  11. Khorana, H.G. (1979) ‘Total synthesis of a gene’, Science, 203, 614–25.CrossRefGoogle Scholar
  12. Lacks, S.A. (1977) ‘Binding and entry of DNA in bacterial transformation’, in J.L. Reissig (ed.), Receptors and Recognition, B3: Microbial Interactions ( Chapman and Hall, London ), pp. 177–232.Google Scholar
  13. Low, K.B. and Porter, D.D. (1978) ‘Modes of gene transfer and recombination in bacteria’, Ann. Rev. Genet., 12, 249–287.CrossRefGoogle Scholar
  14. Old, R.W. and Primrose, S.B. (1980) Principles of Gene Manipulation (Blackwell, Oxford). Roberts, R.J. (1976) ‘Restriction endonucleases’, CRC Crit Rev. Biochem., 4. 123–164.Google Scholar
  15. Roberts, R.J. (1981) ‘Restriction and modification enzymes and their recognition sequences’,Nucl. Acids Res., 9,r75-r96. (This list is updated annually.)Google Scholar
  16. Smith, H.O. (1979) ‘Nucleotide sequence specificity of restriction endonucleases’, Science, 205, 455–462.CrossRefGoogle Scholar
  17. Wu, R. (1978) ‘DNA sequence analysis’, Ann. Rev. Biochem., 47, 607–634.CrossRefGoogle Scholar
  18. Wu, R., Bahl, C.P. and Narang, S.A.C. (1978) ‘Synthetic oligodeoxynucleotides for analysis of DNA structure and function’, Prog. NucL Acid Res. Mol. Biol, 21, 101–141.CrossRefGoogle Scholar

Copyright information

© Robert E. Glass 1982

Authors and Affiliations

  • Robert E. Glass
    • 1
  1. 1.NottinghamUK

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