Molecular Determinants of Damage Recognition by Mammalian Nucleotide Excision Repair
The mechanism by which XPA, RPA, XPC-HHR23B, TFIIH and possibly other factors discriminate a wide range of chemically dissimilar DNA lesions as substrates of mammalian nucleotide excision repair is poorly understood. The striking versatility of nucleotide excision repair led to the assumption that its recognition subunits detect conformational changes imposed on DNA at sites of damage rather than specific base modifications.1-5 As indicated in Figure 7.1, this hypothesis was prompted by the observation that many base lesions alter the helical parameters of DNA by inducing kinks,6,7 bends8 or localized unwinding,9 suggesting that damage-induced conformational distortion may constitute an important determinant of recognition by the nucleotide excision repair system.
KeywordsExcision Repair Nucleotide Excision Repair Double Helix Damage Recognition Base Adduct
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