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Methods, Models, and Analysis of Bacterial Adhesion

  • Itzhak Ofek
  • Ronald J. Doyle

Abstract

It is axiomatic to consider that most living and nonliving surfaces have a tendency to be colonized by microorganisms. The importance of microbial adhesion and colonization to surfaces was not appreciated until molecular techniques were applied to analyze modes and mechanisms of cell—substratum interactions. As more and more techniques became available, new knowledge was gained that made it possible to understand the modulation of the adhesion and subsequent colonization of many microorganisms. To date, no single experimental system has been developed that can be used to adequately characterize all aspects of microbe—substratum interactions. It is therefore essential that the reliabilities, advantages, and limitations of the existing techniques be understood. Most techniques employed in the study of adhesion yield restricted amounts of information, usually about defined events in a complicated series of interactions. This chapter considers methods for the study of adhesion. Consideration is given to model systems, methods for separating adherent from nonadherent cells, controlled and uncontrolled variables in experimental design, and approaches used in analyzing adhesion data. Finally, methods related to the identification and regulation of expression of adhesins and their receptors are reviewed.

Keywords

Bacterial Adhesion Tissue Culture Cell Adherent Bacterium Positive Cooperativity Adhesion Experiment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall, Inc. 1994

Authors and Affiliations

  • Itzhak Ofek
    • 1
  • Ronald J. Doyle
    • 2
  1. 1.Tel-AvivIsrael
  2. 2.LouisvilleUSA

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