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Methods, Models, and Analysis of Bacterial Adhesion

  • Chapter
Bacterial Adhesion to Cells and Tissues

Abstract

It is axiomatic to consider that most living and nonliving surfaces have a tendency to be colonized by microorganisms. The importance of microbial adhesion and colonization to surfaces was not appreciated until molecular techniques were applied to analyze modes and mechanisms of cell—substratum interactions. As more and more techniques became available, new knowledge was gained that made it possible to understand the modulation of the adhesion and subsequent colonization of many microorganisms. To date, no single experimental system has been developed that can be used to adequately characterize all aspects of microbe—substratum interactions. It is therefore essential that the reliabilities, advantages, and limitations of the existing techniques be understood. Most techniques employed in the study of adhesion yield restricted amounts of information, usually about defined events in a complicated series of interactions. This chapter considers methods for the study of adhesion. Consideration is given to model systems, methods for separating adherent from nonadherent cells, controlled and uncontrolled variables in experimental design, and approaches used in analyzing adhesion data. Finally, methods related to the identification and regulation of expression of adhesins and their receptors are reviewed.

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Authors and Affiliations

  1. Tel-Aviv, Israel

    Itzhak Ofek

  2. Louisville, KY, USA

    Ronald J. Doyle

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  1. Itzhak Ofek
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  2. Ronald J. Doyle
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© 1994 Chapman & Hall, Inc.

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Ofek, I., Doyle, R.J. (1994). Methods, Models, and Analysis of Bacterial Adhesion. In: Bacterial Adhesion to Cells and Tissues. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6435-1_2

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  • DOI: https://doi.org/10.1007/978-1-4684-6435-1_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-6437-5

  • Online ISBN: 978-1-4684-6435-1

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