Mechanism of transcription from nif promoters: involvement of IHF

  • E. Santero
  • T. Hoover
  • S. Kustu


In a wide variety of gram-negative bacteria belonging to the phylum “Purple bacteria”, transcription of nitrogen fixation (nif) genes depends on an alternative holoenzyme form of RNA polymerase carrying σ54 as the sigma factor. Sigma 54 confers on core RNA polymerase a unique promoter recognition specificity that consists of an essential GG-N10-GC minimal consensus sequence located 11 to 14 base pairs upstream of transcriptional startsites. This consensus sequence constitutes a binding site for σ54-holoenzyme.


Purple Bacterium Open Complex Crude Cell Extract Promoter Strength Integration Host Factor 
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Copyright information

© Routledge, Chapman & Hall, Inc. 1990

Authors and Affiliations

  • E. Santero
    • 1
  • T. Hoover
    • 1
  • S. Kustu
    • 1
  1. 1.Departments of Plant Pathology and Molecular and Cellular BiologyUniversity of CaliforniaBerkeleyUSA

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