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Molecular analysis of the denitrification system of pseudomonads

  • Walter G. Zumft

Abstract

Bacterial denitrification is an alternative form of energy conservation by facultative anaerobic bacteria in the absence of oxygen. Denitrification may be conceived as the assembly of four, not always tightly coupled respiratory processes, which depend as terminal electron acceptors on nitrate, nitrite, nitric oxide (NO) or nitrous oxide (N2O) (Fig. 1). The process is of importance for ground and waste water management (nitrate removal), agronomy (loss of fixed nitrogen), and atmospheric chemistry (NO and N2O as reactants in the stratosphere). Denitrification has been described for two genera of the halobacteria and about 50 genera of the eubacteria, totalling nearly 130 denitrifying species (35). The trait occurs in the diazotrophic genera Aquaspirillum, Azospirillum, Bradyrhizobium, Pseudomonas, Rhizobium, Rhodobacter, and Rhodopseudomonas, somewhat unexpectedly because antagonistic N-transformations are involved. Denitrification is prevalent among the pseudomonads; they harbor also the dominant denitrifiers worldwide (8). Our objective is the characterization of the process from a single bacterial species.

Keywords

Nitric Oxide Nitrite Reductase Nitrate Removal EcoRI Fragment Pseudomonas Stutzeri 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Routledge, Chapman & Hall, Inc. 1990

Authors and Affiliations

  • Walter G. Zumft
    • 1
  1. 1.Department of MicrobiologyUniversity of KarlsruheKarlsruhe 1Federal Republic of Germany

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