Kinetics and mechanisms of ATP hydrolysis, electron transfers and proton release by Klebsiella pneumoniae nitrogenase

  • Roger N. F. Thorneley


Eady et al. (3) and Hageman et al. (4) used the rapid acid quench technique to demonstrate that the transfer of one electron from the 4Fe-4S centre in the Fe protein to the MoFe protein is coupled to the hydrolysis of two equivalents of MgATP; a stoichiometry also given by the steady state rates of substrate reduction and Pi formation under optimum conditions.


MoFe Protein Rapid Acid Protein Dissociation AFRC Institute Hydrolysis MgATP 
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Copyright information

© Routledge, Chapman & Hall, Inc. 1990

Authors and Affiliations

  • Roger N. F. Thorneley
    • 1
  1. 1.AFRC Institute of Plant Science Research, Nitrogen Fixation LaboratoryUniversity of SussexBrightonUK

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