The Use of Affinity Chromatography in the Purification of Steroid-Binding Proteins
The term affinity chromatography seems to have been first used by Cuatrecasas et al. (1968); however, the principles involved have long been known in the field of immunology. In fact, as early as 1936, Landsteiner and van der Scheer used insolubilized haptens for the isolation of the corresponding antibodies. Since then numerous examples of antibody purification by use of immunosorbents have been reported (Silman and Katchalski, 1966).
KeywordsAffinity Chromatography Maleic Anhydride Cyanogen Bromide Potassium Thiocyanate Trans Cortin
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