The Use of Affinity Chromatography in the Purification of Steroid-Binding Proteins

  • Sumner H. Burstein


The term affinity chromatography seems to have been first used by Cuatrecasas et al. (1968); however, the principles involved have long been known in the field of immunology. In fact, as early as 1936, Landsteiner and van der Scheer used insolubilized haptens for the isolation of the corresponding antibodies. Since then numerous examples of antibody purification by use of immunosorbents have been reported (Silman and Katchalski, 1966).


Affinity Chromatography Maleic Anhydride Cyanogen Bromide Potassium Thiocyanate Trans Cortin 
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Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • Sumner H. Burstein
    • 1
  1. 1.Worcester Foundation for Experimental BiologyShrewsburyUSA

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