Hapten-Radioimmunoassay for Steroid Hormones

  • Gordon D. Niswender
  • A. Rees MidgleyJr.


One of the major advantages of using radioimmunoassay techniques for the measurement of steroid hormones is their unique potential for quantitating these hormones in unextracted serum, providing a truly specific antibody can be obtained to a given steroid. However, data regarding the factors which influence the specificity of antibodies to steroid hormones are incomplete. Since the basic structure of all steroid hormones is similar, i.e., they all contain the same basic cyclopentanophenanthrene nucleus, they offer an unequaled opportunity to study how minor alterations of this basic structure influence their immuno-reactivity. The structure of all steroid hormones differs only in the type and location of functional groups which are attached or introduced into this basic four ring nucleus.


Inhibition Curve Bovine Serum Albumin Molecule Steroid Molecule Steroid Derivative Competitive Protein Binding 
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Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • Gordon D. Niswender
    • 1
  • A. Rees MidgleyJr.
    • 1
  1. 1.The Reproduction and Endocrinology Program, Department of PathologyThe University of MichiganAnn ArborUSA

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