Hapten-Radioimmunoassay for Steroid Hormones
One of the major advantages of using radioimmunoassay techniques for the measurement of steroid hormones is their unique potential for quantitating these hormones in unextracted serum, providing a truly specific antibody can be obtained to a given steroid. However, data regarding the factors which influence the specificity of antibodies to steroid hormones are incomplete. Since the basic structure of all steroid hormones is similar, i.e., they all contain the same basic cyclopentanophenanthrene nucleus, they offer an unequaled opportunity to study how minor alterations of this basic structure influence their immuno-reactivity. The structure of all steroid hormones differs only in the type and location of functional groups which are attached or introduced into this basic four ring nucleus.
KeywordsInhibition Curve Bovine Serum Albumin Molecule Steroid Molecule Steroid Derivative Competitive Protein Binding
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