Radioimmunoassay of Plasma Estrogens: Use of Polymerized Antibodies
Antibodies to estradiol can be produced by immunization of sheep with estradiol-17β-succinyl-bovine serum albumin (Ferin et al., 1968). The biologic potency of these antibodies in neutralizing circulating estrogens has been demonstrated by Ferin et al. (1968, 1969). The availability of steroid antibodies opened the possibility of their use in the development of radioimmunoassay of steroids. Preliminary results of radioimmunoassays of estradiol (Abraham, 1969) and testosterone (Niswender and Midgley, 1969) have been reported recently.
KeywordsMaleic Anhydride Ethyl Chloroformate Plasma Estrogen Antibody Reagent Neutral Steroid
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