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Radioimmunoassay of Plasma Estrogens: Use of Polymerized Antibodies

  • George Mikhail
  • Hsiu-Wu Chung
  • Michel Ferin
  • Raymond L. Vande Wiele

Abstract

Antibodies to estradiol can be produced by immunization of sheep with estradiol-17β-succinyl-bovine serum albumin (Ferin et al., 1968). The biologic potency of these antibodies in neutralizing circulating estrogens has been demonstrated by Ferin et al. (1968, 1969). The availability of steroid antibodies opened the possibility of their use in the development of radioimmunoassay of steroids. Preliminary results of radioimmunoassays of estradiol (Abraham, 1969) and testosterone (Niswender and Midgley, 1969) have been reported recently.

Keywords

Maleic Anhydride Ethyl Chloroformate Plasma Estrogen Antibody Reagent Neutral Steroid 
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References

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Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • George Mikhail
    • 1
  • Hsiu-Wu Chung
    • 1
  • Michel Ferin
    • 2
  • Raymond L. Vande Wiele
    • 2
  1. 1.Department of Obstetrics and GynecologyThe University of Texas, Southwestern Medical School at DallasDallasUSA
  2. 2.Department of Obstetrics and GynecologyColumbia University College of Physicians and SurgeonsNew YorkUSA

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