Solid-Phase Radioimmunoassay of Serum Estradiol-17β: A Semi-Automated Approach
Estrogens, like other steroids, exist in the organism in many forms, the predominant form depending on the particular biologic fluid. In urine, estrogens are almost entirely in the conjugated forms, as sulfates, β-glucuronides, and other less common conjugates. In blood, however, a significant proportion of the estrogens exist in the free (unconjugated) form. Both free and conjugated estrogens can be bound to intra- and extracellular proteins. It is beyond the scope of this presentation to expand further on this aspect of estrogen metabolism. (For review on above subjects, see Preedy, 1968). However, it must be emphasized that a comprehensive study of estrogen metabolism is a formidable task, in view of the large number of estrogens found in biologic materials and the various forms in which they exist.
KeywordsAssay Buffer Estrogen Metabolism Label Tracer Tritium Atom Estrogen Conjugate
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- Berson, S. A., and R. S. Yalow. 1959. Assay of plasma insulin in human subjects by immunological methods. Nature (London), 184:1648.Google Scholar
- Karush, F. 1962. Immunologic specificity and molecular structure. In Taliaferro, W. H., and J. H. Humphrey, eds. Advances in Immunology, Vol. 2, 1–39, New York, Academic Press, Inc.Google Scholar
- Odell, W. D., P. L. Rayford, and G. T. Ross. 1967. Simplified partially automated method for radioimmunoassay of human TSH, GH, LH, and FSH. J. Clin. Med., 70:973–980.Google Scholar
- Preedy, J. R. K. 1968. Estrogens. In Dorfman, R. I., ed. Methods in Hormone Research, 2nd ed. 1–64, New York, Academic Press, Inc.Google Scholar
- Pressman, D., and A. Grossberg. 1968. The Structural Basis of Antibody Specificity. New York, W. A. Benjamin, Inc.Google Scholar