In these three days under an intimate stimulating environment with free interchange of information, we have concerned ourselves with immunologic means of quantifying steroid hormones. Ten years ago Berson and Yalow (1959) and Ekins (1960) first published the principles of competitive binding assays. Since that time the general applicability of these principles has become evident; the procedures may be adopted to quantify any hormonal substance. Experience gained by many investigators in characterizing competitive binding assays for polypeptides has led to increased comprehension of the required properties of such systems (Odell et al., 1969). Antisera have been prepared against progressively smaller hormones: ACTH (MW 4566), gastrin (MW 2096), vasopressin (MW 1080), and angiotensin II (MW 1170). Even though so-called antigenic determinants are believed to be approximately equivalent to 600 to 800 molecular weight substances, smaller haptenic substances have been shown to be capable of being immunogenic, usually when coupled to larger molecules. Thus in the past few years, antibodies have been prepared against steroid-protein conjugates and the steroids themselves have served as haptenic reactants (Erlanger et al., 1957). Several important properties of antisera are of concern to us.
KeywordsLuteinizing Hormone Polypeptide Hormone Competitive Binding Assay Free Hormone Hormonal Substance
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