Abstract
Immunologic methods for steroid determination depend on the ability of antibodies directed against a steroid determinant group to react preferentially with that particular steroid. The specificity of the reaction depends on a complementary fit of the combining site of the antibody with the steroid of interest.
A unit of the New York State Department of Health.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Brenneman, L., and S. J. Singer. 1968. The generation of antihapten antibodies with electrophoretically homogeneous L chains. Proc. Nat. Acad. Sci. USA, 60:258–264.
Edelman, G. M., D. E. Olins, J. A. Gaily, and N. D. Zinder. 1963. Reconstitution of immunologic activity by interaction of polypeptide chains of antibodies. Proc. Nat. Acad. Sci. USA, 50:753–761.
Franek, F., and R. S. Nezlin. 1963. The role of different peptide chains of antibody in the antigen-antibody reaction. Biokhimiia, 28:193–203.
Freedman, M. H., A. L. Grossberg, and D. Pressman. 1968a. Evidence for ammonium and guanidinium groups in the combining sites of anti-p-azobenzenearsonate antibodies—separation of two different populations of antibody molecules. J. Biol. Chem., 243:6186–6195.
--- A. L. Grossberg, and D. Pressman. 1968b. The effects of complete modification of amino groups on the antibody activity of antihapten antibodies. Reversible inactivation with maleic anhydride. Biochemistry (Wash.), 7:1941–1950.
--- A. L. Grossberg, and D. Pressman. 1968c. Participation of amino groups in the active sites of antibodies. Immunochemistry, 5:367–381.
Grossberg, A. L., and D. Pressman. 1960. Nature of the combining site of antibody against a hapten bearing a positive charge. J. Amer. Chem. Soc., 82:5478–5482.
--- and D. Pressman. 1968. Modification of arginine in the active sites of antibodies. Biochemistry (Wash.), 7:272–279.
Koshland, M. E., F. M. Englberger, and S. M. Gaddone. 1965. Evidence against the universality of a tyrosyl residue at antibody combining sites. Immunochemistry, 2:115–125.
Koyama, J., A. L. Grossberg, and D. Pressman. 1968a. Variability among anti-p-azobenzenearsonate antibody preparations as revealed by affinity labeling. Biochemistry (Wash.), 7:1935–1940.
--- A. L. Grossberg, and D. Pressman. 1968b. Conformational changes in anti-p-azobenzenearsonate antibody caused by iodination. Biochemistry (Wash.), 7:2369–2375.
Little, E. R., and H. N. Eisen. 1967. Evidence for tryptophan in the active sites of antibodies to polynitrobenzenes. Biochemistry (Wash.), 6:3119–3125.
Mattioli, C. A., Y. Yagi, and D. Pressman. 1968. Production and properties of mouse antihapten antibodies. J. Immun., 101:939–948.
Nisonoff, A., S. Zappacosta, and R. Jureziz. 1967. Properties of crystallized rabbit anti-p-azobenzoate antibody. Cold Spring Harbor Symp. Quant. Biol., 32:89–93.
Parker, C. W., T.-J. Yoo, M. C. Johnson, and S. M. Godt. 1967a. Fluorescent probes for the study of the antibody-hapten reaction. I. Binding of the 5-dimethylaminonaphthalene-1-sulfonamido group by homologous rabbit antibody. Biochemistry (Wash.), 6:3408–3416.
--- S. M. Godt, and M. C. Johnson. 1967b. Fluorescent probes for the study of the antibody-hapten reaction. II. Variation in the antibody combining site during the immune response. Biochemistry (Wash.), 6:3417–3427.
Pressman, D., and A. L. Grossberg. 1968. The Structural Basis of Antibody Specificity. New York, W. A. Benjamin, Inc.
Roholt, O. A., A. L. Grossberg, and D. Pressman. 1968. Limited heterogeneity of antibodies. Fed. Proc., 27:684.
--- and D. Pressman. 1968. Structural differences between antibodies against the same hapten by individual rabbits. Immunochemistry, 5:265–275.
--- G. Radzimski, and D. Pressman. 1965a. Preferential recombination of antibody chains to form effective binding sites. J. Exp. Med., 122:785–798.
--- G. Radzimski, and D. Pressman. 1965b. Polypeptide chains of antibody: Effective binding sites require specificity in combination. Science, 147:613–615.
Singer, S. J., and R. F. Doolittle. 1966. Antibody active sites and immunoglobulin molecules. Science, 153:13–24.
Velick, S. F., C. W. Parker, and H. N. Eisen. 1960. Excitation energy transfer and the quantitative study of the antibody hapten reaction. Proc. Nat. Acad. Sci. USA, 46:1470–1482.
Winkler, M. H. 1962. A molecular probe for the antibody site. J. Molec. Biol., 4:118–120.
Yoo, T.-J., and C. W. Parker. 1968. Fluorescent enhancement in antibody-hapten interaction 1-anilinonaphthalene-8-sulfonate as a fluorescent molecular probe for anti-azonaphthalene sulfonate antibody. Immunochemistry, 5:143–153.
--- O. A. Roholt, and D. Pressman. 1967. Specific binding activity of isolated light chains of antibodies. Science, 157:707–709.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1970 Plenum Press, New York
About this chapter
Cite this chapter
Pressman, D., Grossberg, A.L. (1970). Structural Basis of Antibody Specificity. In: Péron, F.G., Caldwell, B.V. (eds) Immunologic Methods in Steroid Determination. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6051-3_1
Download citation
DOI: https://doi.org/10.1007/978-1-4684-6051-3_1
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-6053-7
Online ISBN: 978-1-4684-6051-3
eBook Packages: Springer Book Archive