Structural Basis of Antibody Specificity

  • David Pressman
  • Allan L. Grossberg


Immunologic methods for steroid determination depend on the ability of antibodies directed against a steroid determinant group to react preferentially with that particular steroid. The specificity of the reaction depends on a complementary fit of the combining site of the antibody with the steroid of interest.


Light Chain Nitro Group Individual Animal Maleic Anhydride Antibody Molecule 
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  1. Brenneman, L., and S. J. Singer. 1968. The generation of antihapten antibodies with electrophoretically homogeneous L chains. Proc. Nat. Acad. Sci. USA, 60:258–264.PubMedCrossRefGoogle Scholar
  2. Edelman, G. M., D. E. Olins, J. A. Gaily, and N. D. Zinder. 1963. Reconstitution of immunologic activity by interaction of polypeptide chains of antibodies. Proc. Nat. Acad. Sci. USA, 50:753–761.PubMedCrossRefGoogle Scholar
  3. Franek, F., and R. S. Nezlin. 1963. The role of different peptide chains of antibody in the antigen-antibody reaction. Biokhimiia, 28:193–203.Google Scholar
  4. Freedman, M. H., A. L. Grossberg, and D. Pressman. 1968a. Evidence for ammonium and guanidinium groups in the combining sites of anti-p-azobenzenearsonate antibodies—separation of two different populations of antibody molecules. J. Biol. Chem., 243:6186–6195.PubMedGoogle Scholar
  5. --- A. L. Grossberg, and D. Pressman. 1968b. The effects of complete modification of amino groups on the antibody activity of antihapten antibodies. Reversible inactivation with maleic anhydride. Biochemistry (Wash.), 7:1941–1950.CrossRefGoogle Scholar
  6. --- A. L. Grossberg, and D. Pressman. 1968c. Participation of amino groups in the active sites of antibodies. Immunochemistry, 5:367–381.PubMedCrossRefGoogle Scholar
  7. Grossberg, A. L., and D. Pressman. 1960. Nature of the combining site of antibody against a hapten bearing a positive charge. J. Amer. Chem. Soc., 82:5478–5482.CrossRefGoogle Scholar
  8. --- and D. Pressman. 1968. Modification of arginine in the active sites of antibodies. Biochemistry (Wash.), 7:272–279.CrossRefGoogle Scholar
  9. Koshland, M. E., F. M. Englberger, and S. M. Gaddone. 1965. Evidence against the universality of a tyrosyl residue at antibody combining sites. Immunochemistry, 2:115–125.PubMedCrossRefGoogle Scholar
  10. Koyama, J., A. L. Grossberg, and D. Pressman. 1968a. Variability among anti-p-azobenzenearsonate antibody preparations as revealed by affinity labeling. Biochemistry (Wash.), 7:1935–1940.CrossRefGoogle Scholar
  11. --- A. L. Grossberg, and D. Pressman. 1968b. Conformational changes in anti-p-azobenzenearsonate antibody caused by iodination. Biochemistry (Wash.), 7:2369–2375.CrossRefGoogle Scholar
  12. Little, E. R., and H. N. Eisen. 1967. Evidence for tryptophan in the active sites of antibodies to polynitrobenzenes. Biochemistry (Wash.), 6:3119–3125.CrossRefGoogle Scholar
  13. Mattioli, C. A., Y. Yagi, and D. Pressman. 1968. Production and properties of mouse antihapten antibodies. J. Immun., 101:939–948.PubMedGoogle Scholar
  14. Nisonoff, A., S. Zappacosta, and R. Jureziz. 1967. Properties of crystallized rabbit anti-p-azobenzoate antibody. Cold Spring Harbor Symp. Quant. Biol., 32:89–93.CrossRefGoogle Scholar
  15. Parker, C. W., T.-J. Yoo, M. C. Johnson, and S. M. Godt. 1967a. Fluorescent probes for the study of the antibody-hapten reaction. I. Binding of the 5-dimethylaminonaphthalene-1-sulfonamido group by homologous rabbit antibody. Biochemistry (Wash.), 6:3408–3416.CrossRefGoogle Scholar
  16. --- S. M. Godt, and M. C. Johnson. 1967b. Fluorescent probes for the study of the antibody-hapten reaction. II. Variation in the antibody combining site during the immune response. Biochemistry (Wash.), 6:3417–3427.CrossRefGoogle Scholar
  17. Pressman, D., and A. L. Grossberg. 1968. The Structural Basis of Antibody Specificity. New York, W. A. Benjamin, Inc.Google Scholar
  18. Roholt, O. A., A. L. Grossberg, and D. Pressman. 1968. Limited heterogeneity of antibodies. Fed. Proc., 27:684.Google Scholar
  19. --- and D. Pressman. 1968. Structural differences between antibodies against the same hapten by individual rabbits. Immunochemistry, 5:265–275.PubMedCrossRefGoogle Scholar
  20. --- G. Radzimski, and D. Pressman. 1965a. Preferential recombination of antibody chains to form effective binding sites. J. Exp. Med., 122:785–798.PubMedCrossRefGoogle Scholar
  21. --- G. Radzimski, and D. Pressman. 1965b. Polypeptide chains of antibody: Effective binding sites require specificity in combination. Science, 147:613–615.PubMedCrossRefGoogle Scholar
  22. Singer, S. J., and R. F. Doolittle. 1966. Antibody active sites and immunoglobulin molecules. Science, 153:13–24.PubMedCrossRefGoogle Scholar
  23. Velick, S. F., C. W. Parker, and H. N. Eisen. 1960. Excitation energy transfer and the quantitative study of the antibody hapten reaction. Proc. Nat. Acad. Sci. USA, 46:1470–1482.PubMedCrossRefGoogle Scholar
  24. Winkler, M. H. 1962. A molecular probe for the antibody site. J. Molec. Biol., 4:118–120.PubMedCrossRefGoogle Scholar
  25. Yoo, T.-J., and C. W. Parker. 1968. Fluorescent enhancement in antibody-hapten interaction 1-anilinonaphthalene-8-sulfonate as a fluorescent molecular probe for anti-azonaphthalene sulfonate antibody. Immunochemistry, 5:143–153.PubMedCrossRefGoogle Scholar
  26. --- O. A. Roholt, and D. Pressman. 1967. Specific binding activity of isolated light chains of antibodies. Science, 157:707–709.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • David Pressman
    • 1
  • Allan L. Grossberg
    • 1
  1. 1.Roswell Park Memorial InstituteBuffaloUSA

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