Abstract
In order to study the structure of a complex of monomeric actin and myosin head (S1) in the presence of nucleotide, non-polymerizable G-actin was prepared by the reaction of G-actin with m-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS) and diazonium(lH)tetrazole (DHT). Although singly-modified MBS-actin forms a 2:1 complex with S1, doubly-modified DHT/MBS-G-actin forms a 1:1 complex with a dissociation constant of ∼10−6 M and can activate Mg-ATPase of S1 by 2-fold. Using a synchrotron X-ray source, we have measured X-ray solution scattering from reversible acto-S1 complexes obtained by mixing rabbit skeletal S1 and DHT/MBS-G-actin in the absence of nucleotide or in the presence of MgADP. The Guinier plots of the scattering intensity showed straight lines indicating no large aggregates and gave the radii of gyration of the complexes to be 49 and 46 Å in the absence and presence of ADP, respectively. The analysis of the scattering curves showed that a monomeric actin binds to a tip of S1, and that the complex undergoes substantial rearrangement resulting in a more compact structure when it binds ADP.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Kabsch, W., Mannherz, H. G., Suck, D., Pai, E. F. & Holmes, K. C. Nature, 347, 37–44 (1990).
Rayment, I., Rypneiwski, W. R., Schmidt-Base, K., Smith, R., Tomchick, D. R., Benning, M. M., Winkelmann, D. A., Wesenberg, G. & Holden, H. M. Science, 261, 58–65 (1993).
Rayment, I., Holden, H. M., Whittacker, M., Yohn, C. B., Lorenz, M., Holmes, K. C. & Milligan, R. A. Science, 261, 58–65 (1993).
Schroeder, R. R., Manstein, D. J., Jahn, W., Holden, H. M., Rayment, I., Holmes, K. C. & Spudich, J. A. Nature, 364, 171–174 (1993).
Mendelson, R. A. & Morris, E. P. Proc. Natn. Acad. Sci. U.S.A. 94, 8533–8538 (1997).
Chaussepied, P. & Kasprzak, A. A. Nature, 342, 950–953 (1989).
Miki, M., Barden, J. A., dos Remedios, C. G., Phillips, L. & Hambly, B. D. Eur. J. Biochem. 165, 125–130 (1987).
Bettache, N., Bertrand, R. & Kassab, R. Proc. Natn. Acad. Sci. U.S.A. 86, 6028–6032 (1989).
Valentin-Ranc, C., Combeau, C., Pantaloni, D. & Carlier, M.-F. J. Biol. Chem. 266, 17872–17879 (1991).
Arata, T. J. Biochem. Tokyo, 109, 335–340 (1991).
Arata, T. Biochemistry, 35, 16061–16068 (1996).
Heinz, D. & Faulstich, H. Biochemistry, 35, 258–265 (1996).
Bettache, N., Bertrand, R. & Kassab, R. Biochemistry, 29, 9085–9091 (1990).
Lheureux, K., Forne, T. & Chaussepied, P. Biochemistry, 32, 10005–10014 (1993).
Bender, N., Fasold, H., Kenmoku, A., Midelhoff, G. & Volk, K.-E. Eur. J. Biochem. 64, 215–218 (1976).
Wakabayashi, K., Tokunaga, M., Kohno, M., Sugimoto, Y. Hamanaka, T., Takezawa, Y., Wakabayashi, T. & Amemiya, Y. Science, 258, 443–447 (1992).
Sugimoto, Y., Tokunaga, M., Takezawa, Y., Ikebe, M. & Wakabayashi, K. Biophys. J. 68, 29s–34s (1995).
Amemiya, Y., Wakabayashi, K., Hamanaka, T., Wakabayashi, T., Hashizume, H. & Matsushita, T. Nucl. In-strum. Methods. 208, 471–477 (1983).
Arata, T., Iwasaki, N., Takezawa, Y., Sugimoto, Y. & Wakabayashi, K. Photon Factory Activity Report 1995, 13, 296 (1996).
Solves, J. A., Iniesta, A. & Galcia de Torre, J. Int. Biol. Macromol. 10, 39–43 (1988).
Arata, T. Biophys. J. 68, 325s (1995).
Kimura, T., Arata, T., Sugimoto, Y., Takezawa, Y. & Wakabayashi, K. J. Muscle Res. Cell Motil. Abstr. (in the press).
Holmes, K. C., Popp, D., Gebherd, W. & Kabsch, W. Nature, 347, 44–49 (1990).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Plenum Press, New York
About this chapter
Cite this chapter
Arata, T., Kimura, S., Sugimoto, Y., Takezawa, Y., Iwasaki, N., Wakabayashi, K. (1998). Structure of the Monomeric Actin-Myosin Head Complex as Revealed by X-Ray Solution Scattering. In: Sugi, H., Pollack, G.H. (eds) Mechanisms of Work Production and Work Absorption in Muscle. Advances in Experimental Medicine and Biology, vol 453. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6039-1_9
Download citation
DOI: https://doi.org/10.1007/978-1-4684-6039-1_9
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-6041-4
Online ISBN: 978-1-4684-6039-1
eBook Packages: Springer Book Archive