Measurement of ATP Turnover during Shortening and Lengthening of Rabbit Psoas Myofibrils Using a Fluorescent ATP analog

  • Shigeru Chaen
  • Ibuki Shirakawa
  • Clive R. Bagshaw
  • Haruo Sugi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 453)


In order to study ATP turnover during shortening and lengthening of rabbit psoas myofibrils, we have used fluorescence microscopy in which the displacement of a fluorescent nucleotide analog, 2’(3’)-0-[N-[2-[[Cy3] amido] ethyl] carbamoyl]-adenosine 5′ triphosphate (Cy3-EDA-ATP) bound to cross-bridge on flash photolysis of caged ATP was measured [Chaen et al. (1997) Biophys. J. 73, 2033–2042]1. In the previous paper1, we reported that when a myofibril was imposed to shorten with a constant velocity by a piezoelectric actuator, the nucleotide displacement rate constant initially increased to 0.7 s-1 with increasing shortening velocity and then declined with a further increase in shortening velocity. The rate constant during lengthening measured in the present experiment was found to be not significantly affected. These results suggest that the cross-bridge kinetics show a asymmetrical depenence on the mechanical strain in the cross-bridges, namely, the rate constants are not significantly affected at higher strain during lengthening but depend on the lower strain during shortening.


Actin Filament Thin Filament Isometric Force Sarcomere Length Myosin Head 
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Copyright information

© Plenum Press, New York 1998

Authors and Affiliations

  • Shigeru Chaen
    • 1
  • Ibuki Shirakawa
    • 1
  • Clive R. Bagshaw
    • 2
  • Haruo Sugi
    • 1
  1. 1.Department of Physiology School of MedicineTeikyo UniversityTokyo 173Japan
  2. 2.Department of BiochemistryUniversity of LeicesterLeicesterUK

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