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Distinct Kinetic Properties of Cardiac Myosin Isoforms Revealed by In Vitro Studies

  • Seiryo Sugiura
  • Naoshi Kobayakawa
  • Hideo Fujita
  • Shin-ichi Momomura
  • Shigeru Chaen
  • Haruo Sugi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 453)

Abstract

To clarify the physiological significance of myosin isoform redistribution in cardiac adaptation process, we compared the kinetic property of the two cardiac myosin isoforms using in vitro motility assay techniques. Cardiac myosin isoforms V1 and V3 were obtained from ventricular muscle of young rats and hypothyroid rats respectively. On each of these myosin isoforms fixed on a glass coverslip, fluorescently labeled actin filaments were made to slide in the presence of ATP. To measure the force generated by actomyosin interaction, a small latex bead was attached to the barbed end of an actin filament and the bead was captured by the laser optical trap installed in a microscope. The force was determined from the distance between the bead and the trap positions under either auxotonic or isometric conditions. The time-averaged force generated by multiple cross-bridges did not differ significantly between the two isoforms. On the other hand, the unitary force measurement revealed the same level of amplitude but a longer duration for V3 isoform. The same level of time-averaged force is in agreement with not only our previous finding but the results of maximum force measurement in muscle preparations. The difference in kinetic characteristics of the two isoforms could account for the difference in economy of force development and the basis for cardiac adaptation mechanism.

Keywords

Actin Filament Glass Coverslip Isometric Force Myosin Head Duty Ratio 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1998

Authors and Affiliations

  • Seiryo Sugiura
    • 1
  • Naoshi Kobayakawa
    • 1
  • Hideo Fujita
    • 1
  • Shin-ichi Momomura
    • 1
  • Shigeru Chaen
    • 2
  • Haruo Sugi
    • 2
  1. 1.The Second Department of Internal Medicine School of MedicineUniversity of TokyoBunkyo-ku, Tokyo 113Japan
  2. 2.Department of PhysiologyTeikyo University School of MedicineItabashi-ku, Tokyo 173Japan

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