Effects of v-H-ras on Immortalized Non-Tumorigenic Human Mammary Epithelial Cells
Oncogenes are genes implicated in carcinogenesis which have been identified by virtue of their association with oncogenic viruses, with tumour-specific chromosomal abnormalities and with DNA sequences that transform cultured cells to a tumorigenic state (Bishop, 1985). Activated ras oncogenes have been detected in a wide variety of human cancers (Bos, 1989) and expression of activated ras oncogenes or overexpression of the normal ras proto-oncogenes stimulates proliferation and induces transformation in a number of cell lines (Barbacid, 1987). Recent studies however demonstrated that ras oncogenes can promote either tumorigenic transformation or growth inhibition depending on the phenotype of the cell in which they are expressed. Thus, expression of v-H-ras oncogene leads to growth arrest of mouse Schwann cells (Ridley et al., 1988), rat embryo fibroblasts (Hirakawa and Ruley, 1988) and human thyroid epithelial cells (Wynford-Thomas et al., 1990). These studies were performed using cell lines expressing a temperature-sensitive mutant of large T antigen at the restrictive temperature; it was found that in the presence of a functional T antigen ras induced tumorigenicity, but in its absence, cell growth was inhibited.
KeywordsMammary Epithelial Cell Luminal Cell Human Mammary Epithelial Cell Luminal Epithelial Cell Mammary Epithelial Cell Line
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