Evidence for ras-Dependent and Independent Pathways in NGF-Induced Neuronal Differentiation of PC12 Cells

  • József Szeberényi
  • Hong Cai
  • Geoffrey M. Cooper
Part of the NATO ASI Series book series (NSSA, volume 220)


PC12 cells expressing a dominant inhibitory mutant Ha-Ras protein [p21 (Asn-17) Ha-ras] were used to study signaling pathways involved in neuronal differentiation. Expression of the mutant protein completely blocks NGF-induced neurite outgrowth in these subclones. In contrast, NGF is able to potentiate the effect of dibutyryl cyclic AMP or ionomycin in inducing process formation in PC12 cells expressing the mutant Ras protein. These results suggest that (i) a Ras-independent signaling pathway exists in PC12 cells that is not sufficient for, but can contribute to NGF-induced neuritogenesis; (ii) the block of neuronal differentiation by the mutant Ras protein can be bypassed by either one of two second messengers, cyclic AMP and Ca++.


PC12 Cell Nerve Growth Factor Neurite Outgrowth Neuronal Differentiation Early Response Gene 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • József Szeberényi
    • 1
  • Hong Cai
    • 2
  • Geoffrey M. Cooper
    • 2
  1. 1.Department of BiologyUniversity Medical School of PécsHungary
  2. 2.Dana-Farber Cancer InstituteBostonUSA

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