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Search for Correlations between K-ras Oncogene Activation and Pathology, in Sporadic and Familial Colonic Adenomas

  • J. C. Chomel
  • S. Grandjouan
  • D. A. Spandidos
  • M. Tuilliez
  • C. Bognel
  • A. Kitzis
  • J. C. Kaplan
  • A. Haliassos
Part of the NATO ASI Series book series (NSSA, volume 220)

Abstract

The activation of K-ras oncogene by point mutation at its 12th codon has been reported as a molecular marker of tumor progression in colonic neoplasias (early event, correlated with the increasing size of adenomas, but not with dysplasia)1. The overall frequency of K-ras point mutations has probably been underestimated by the usual former techniques. Direct sequencing of PCR amplified K-ras oncogene demonstrated that 75% and 65% adenomas and adenocarcinomas, respectively, harbored a mutated allele of K-ras at codon 122. We described a new sensitive method for an easier detection of this mutation, based upon the introduction of an artificial restriction site in a modified primer for selective in vitro amplification3. This sensitive method, connected with the feasibility of PCR amplification of DNA in formalin-fixed and paraffin-embedded tissues, prompted us to reevaluate the frequency of K-ras point mutations at codon 12 in both sporadic and familial colonic adenomas.

Keywords

Nucleic Acid Research Familial Polyposis Coli Colonic Neoplasia Homozygous Wild Type Sporadic Adenoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • J. C. Chomel
    • 1
  • S. Grandjouan
    • 2
  • D. A. Spandidos
    • 4
  • M. Tuilliez
    • 3
  • C. Bognel
    • 5
  • A. Kitzis
    • 1
  • J. C. Kaplan
    • 1
  • A. Haliassos
    • 1
    • 4
  1. 1.Institut de Pathologie MoléculaireFrance
  2. 2.Service de GastroenterologieFrance
  3. 3.Service de PathologieCHU COCHINParisFrance
  4. 4.Institute of Biological Research and BiotechnologyNational Hellenic Research FoundationAthensGreece
  5. 5.Institut Gustave RoussyVillejuif CedexFrance

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