Increases in Phospholipase A2 Activity and in the ADP Ribosylation of G Proteins in K-ras-Transformed Thyroid Cells

  • Daniela Corda
  • Salvatore Valitutti
  • Luisa Iacovelli
  • Maria Di Girolamo
Part of the NATO ASI Series book series (NSSA, volume 220)


Epithelial cell lines derived from rat thyroid have been employed to analyze the effects of ras-induced transformation on the signal pathways regulating growth and differentiation.


Pertussis Toxin Thyroid Cell Arachidonic Acid Release FRTL5 Cell Normal Thyroid Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    F.S. Ambesi-Impiombato, and H. Perrild eds FRTL5 today. Elsevier Science Publishers, Amsterdam, (1989).Google Scholar
  2. 2.
    F. S. Ambesi-Impiombato, R. Picone, and D. Tramontano, Influence of hormones and serum on growth and differentiation of the thyroid cell strain FRTL, Cold Spring Harbor Symp. Quant. Biol. 9:483–492 (1982).Google Scholar
  3. 3.
    W.A. Valente, P. Vitti, L.D. Kohn, M.L. Brandi, C.M. Rotella, R. Toccafondi, D. Tramontano, S.M. Aloj and F.S. Ambesi-Impiombato, The relationship of growth and adenylate cyclase activity in cultured thyroid cells: separate bioeffects of thyrotropin, Endocrinology 112:71–79 (1983).PubMedCrossRefGoogle Scholar
  4. 4.
    A. Fusco, A. Pinto, F.S. Ambesi-Impiombato, G. Vecchio, and N. Tsuchida, Transformation of rat thyroid epithelial cells by kirsten murine sarcoma virus, Int. J. Cancer 28:655–662 (1981).PubMedCrossRefGoogle Scholar
  5. 5.
    A. Fusco, A. Pinto, D. Tramontano, G. Tajana, G. Vecchio, and N. Tsuchida, Block in the expression of differentiation markers of rat thyroid epithelial cells by transformation with kirsten murine sarcoma virus, Cancer Res., 42:618–626 (1982).PubMedGoogle Scholar
  6. 6.
    G. Colletta, A. Pinto, P.P. Di Fiore, A. Fusco, M. Ferrentino, V.E. Avvedimento, N. Tsuchida, and G. Vecchio, Dissociation between transformed and differentiated phenotype in rat thyroid epithelial cells after transformation with a temperature-sensitive mutant of the kirsten murine sarcoma virus, Mol. Cell. Biol. 3:2099–2109 (1983).PubMedGoogle Scholar
  7. 7.
    G. Colletta, D. Corda, G. Schettini, A.M. Cirafici, L.D. Kohn, and E. Consiglio, Adenylate cyclase activity v-ras-k transformed rat epithelial thyroid cells FEBS Lett. 228:37–41 (1988).PubMedCrossRefGoogle Scholar
  8. 8.
    S. Valitutti, P. Cucchi, G. Colletta, C. Di Filippo, and D. Corda, Transformation by the K-ras oncogene correlates with increases in phospholipase A2 activity, glycerophosphoinositol production and phosphoinositide synthesis in thyroid cells, Cell. Signal., in press (1991).Google Scholar
  9. 9.
    M. Di Girolamo, D. D’Arcangelo, P. Gierschik, and D. Corda, Characterization of trimeric G proteins in normal and k-ras transformed cells. Cell Biol. Int. Rep., 14S:45 (1990).CrossRefGoogle Scholar
  10. 10.
    M. Di Girolamo, D. D’Arcangelo, P. Gierschik, and D. Corda, Increased ADP- ribosylation of trimeric G proteins in k-ras transformed cells, (submitted)Google Scholar
  11. 11.
    D. Bar-Sagi, and J.R. Feramisco, Induction of membrane ruffling and fluid-phase pinocytosis in quiescent fibroblasts by ras proteins Science 233:1061–1068 (1986).PubMedCrossRefGoogle Scholar
  12. 12.
    D. Corda, C. Marcocci, L.D. Kohn, J. Axelrod, and A. Luini, Association of the changes in cytosolic Ca2+ and iodide efflux induced by thyrotropin and by the stimulation of α1-adrenergic receptors in cultured rat thyroid cell, J. Biol. Chem. 260:9230–9236 (1985).PubMedGoogle Scholar
  13. 13.
    D. Corda, and L.D. Kohn, Role of pertussis toxin sensitive G proteins in the alpha-1 adrenergic receptor but not in the thyrotropin receptor mediated activation of membrane phospholipases and iodide fluxes in FRTL-5 thyroid cells, Biochem. Biophys. Res. Commun. 141:1000–1006 (1986).PubMedCrossRefGoogle Scholar
  14. 14.
    C. Bizzarri, M. Di Girolamo, M.C. D’Orazio, D. Corda, Evidence that a guanine nucleotide-binding protein linked to a muscarinic receptor inhibits directly phospholipase C., Proc. Natl. Acad. Sci. USA 87:4889–4893 (1990).PubMedCrossRefGoogle Scholar
  15. 15.
    R.M. Burch, A. Luini, and J. Axelrod, Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to α1-adrenergic stimulation in FRTL5 thyroid cells, Proc. Natl. Acad. Sci. USA 83:7201–7205 (1986).PubMedCrossRefGoogle Scholar
  16. 16.
    D. Corda, L. Iacovelli, and M. Di Girolamo, Coupling of the α1-adrenergic and thyrotropin receptors to second messenger systems in thyroid cells. Role of G-proteins. In: Maggi M, Johnston CA (eds) Horizons in Endocrinology. Raven Press, New York, 169–180 (1988).Google Scholar
  17. 17.
    D. Corda, C. Bizzarri, M. Di Girolamo, S. Valitutti, and A. Luini, G protein-linked receptors in the thyroid, Adv. Exp. Med. Biol. 2 61:245–269 (1989).Google Scholar
  18. 18.
    D. Corda, R.D. Sekura, and L.D. Kohn, Thyrotropin effect on the availability of Ni regulatory protein in FRTL-5 rat thyroid cells to ADP-ribosylation by pertussis toxin, Eur. J. Biochem. 166:475–481 (1987).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Daniela Corda
    • 1
  • Salvatore Valitutti
    • 1
  • Luisa Iacovelli
    • 1
  • Maria Di Girolamo
    • 1
  1. 1.Laboratory of Cellular and Molecular Endocrinology, Istituto di Ricerche Farmacologiche “Mario Negri”Consorzio Mario Negri SudS. Maria ImbaroItaly

Personalised recommendations