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Scintillation Proximity Enzyme Assay a Rapid and Novel Assay Technique Applied to HIV Proteinase

  • Neil D. Cook
  • Robert A. Jessop
  • Philip S. Robinson
  • Anthony D. Richards
  • John Kay
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)

Abstract

The aspartic proteinase encoded by the HIV virus is required for the processing of the structural gag and enzymatic pol precursor proteins1 and is therefore essential for the generation of mature virus particles.2 The proteinase itself is autocatalytically released from the gag-pol fusion precursor to yield an 11 kDa active species.3 This crucial role in the life cycle of the HIV virus has identified this aspartic proteinase as a target for therapeutic intervention to inhibit disease progression.4

Keywords

Scintillation Counter Aspartic Proteinase Suspension Polymerization Assay Tube Scintillation Proximity Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Neil D. Cook
    • 1
  • Robert A. Jessop
    • 1
  • Philip S. Robinson
    • 1
  • Anthony D. Richards
    • 2
  • John Kay
    • 2
  1. 1.Assay Development GroupAmersham International plcCardiffUK
  2. 2.Department of BiochemistryUniversity of Wales College of CardiffCardiffUK

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