Scintillation Proximity Enzyme Assay a Rapid and Novel Assay Technique Applied to HIV Proteinase
The aspartic proteinase encoded by the HIV virus is required for the processing of the structural gag and enzymatic pol precursor proteins1 and is therefore essential for the generation of mature virus particles.2 The proteinase itself is autocatalytically released from the gag-pol fusion precursor to yield an 11 kDa active species.3 This crucial role in the life cycle of the HIV virus has identified this aspartic proteinase as a target for therapeutic intervention to inhibit disease progression.4
KeywordsScintillation Counter Aspartic Proteinase Suspension Polymerization Assay Tube Scintillation Proximity Assay
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